Calpastatin gene polymorphism is associated with rabbit meat quality traits

Figures & data

Figure 1. Sequencing map of three genotypes of the rabbit CAST gene in the intron 3 region.

Table 1. Genotypic and allelic frequencies, χ² value test and diversity parameter for the CAST gene in rabbits.

Breed (number)	Genotype/number/GF^a			Allele/AF^b		χ^2 (HWE)^c	He^d	Ne^e	PIC^f
Hyla (138)	CC/8/0.0580	TT/80/0.5797	CT/50/0.3623	C/0.2391	T/0.7609	0.3639/P > .05	0.3639	1.5721	0.2977
Champagne (139)	18/0.1295	52/0.3741	69/0.4964	0.3777	0.6223	0.4701/P > .05	0.4701	1.8871	0.3596
Tianfu Black (88)	14/0.1591	40/0.4545	34/0.3864	0.3523	0.6477	0.4564/P > .05	0.4564	1.8394	0.3522
Total rabbit (365)	40/0.1096	172/0.4712	153/0.4192	0.3192	0.6808	0.4346/P > .05	0.4346	1.7687	0.3402

^aGF: genotypic frequency.

^bAllelic frequency.

^cχ² (HWE): Hardy–Weinberg equilibrium χ² value. HWE (P > .05), Hardy–Weinberg disequilibrium (P < .05).

^dHe: gene heterozygosity.

^eNe: effective allele number.

^fPIC: polymorphism information content.

Table 2. Least square means for CAST genotype effects on meat pH, colour, and IMF traits in rabbit longissimus dorsi muscle.

	CAST genotype^a
Trait^b	CC	TT	CT	P-value
pH0h	6.47 ± 0.08	6.72 ± 0.08	6.32 ± 0.05	.176
pH24h	5.71 ± 0.03	5.71 ± 0.03	5.74 ± 0.02	.132
	47.70 ± 1.04	49.38 ± 1.03	48.63 ± 0.68	.304
	56.87 ± 0.75	60.23 ± 1.10	58.32 ± 0.71	.264
	4.77 ± 0.41	5.14 ± 0.32	5.67 ± 0.27	.144
	4.02 ± 0.23	4.77 ± 0.44	4.40 ± 0.47	.137
	1.39 ± 0.19^c	3.09 ± 0.11^a	2.81 ± 0.13^ab	.037
	4.62 ± 0.20^c	5.40 ± 0.22^a	5.20 ± 0.23^ab	.048
IMF (%)	2.52 ± 0.11^a	1.49 ± 0.09^b	1.73 ± 0.21^ab	.029

^aValues with different superscripts within the same row differ significantly at P < .05 (a, b) and P < .01 (a, b, c).

^bL* = Lightness from 0 (black) to 100 (white); a* = redness from −60 (green) to 60 (red); b* = yellowness from −60 (blue) to 60 (yellow).

Table 3. Least square means for CAST genotype effects on meat pH, colour, and IMF traits in rabbit biceps femoris muscle.

	CAST genotype^a
Trait^b	CC	TT	CT	P-value
pH0h	6.58 ± 0.14	6.52 ± 0.26	6.18 ± 0.18	.226
pH24h	5.73 ± 0.05	5.72 ± 0.04	5.77 ± 0.02	.125
	52.79 ± 0.80	52.70 ± 0.86	52.94 ± 0.77	.312
	60.93 ± 1.01	62.39 ± 0.92	62.32 ± 0.87	.208
	3.32 ± 0.35	4.15 ± 0.21	3.92 ± 0.33	.114
	4.55 ± 0.23	4.89 ± 0.31	4.53 ± 0.33	.272
	2.05 ± 0.16	2.51 ± 0.19	2.69 ± 0.21	.119
	5.52 ± 0.28	5.17 ± 0.21	5.98 ± 0.31	.071
IMF (%)	3.29 ± 0.31^a	1.68 ± 0.13^b	2.88 ± 0.23^a	.038

^aValues with different superscripts within the same row differ significantly at P < .01 (a, b, c).

^bL* = Lightness from 0 (black) to 100 (white); a* = redness from −60 (green) to 60 (red); b* = yellowness from −60 (blue) to 60 (yellow).