Effect of organic acid blend and Bacillus subtilis alone or in combination on growth traits, blood biochemical and antioxidant status in broilers exposed to Salmonella typhimurium challenge during the starter phase

Figures & data

Table 1. Dietary composition of broiler chicks during starter.

Ingredients	Starter
Yellow corn	57.62
Soybean meal	35.24
Corn oil	2.37
Dicalcium phosphate	2.30
Ground limestone	0.83
Choline chloride	0.05
dl-Methionine	0.20
l-Lysine	0.20
Salt	0.46
Threonine	0.11
Vitamins and minerals premix^a	0.50
Chemical analysis
ME (kcal/kg)	3000
Crude protein (%)	22.0
Non-phytate P (%)	0.50
Calcium (%)	1.05
Lysine (%)	1.30
Methionine (%)	0.55
Sulphur amino acids (%)	0.90
Threonine (%)	0.95

^aVitamin–mineral premix contains the following per kg: vitamin A, 2,400,000 IU; vitamin D, 1,000,000 IU; vitamin E, 16,000 IU; vitamin K, 800 mg; vitamin B1, 600 mg; vitamin B₂, 1600 mg; vitamin B₆, 1000 mg; vitamin B₁₂, 6 mg; niacin, 8000 mg; folic acid, 400 mg; pantothenic acid, 3000 mg; biotin 40 mg; antioxidant, 3000 mg; cobalt, 80 mg; copper, 2000 mg; iodine, 400 mg; iron, 1200 mg; manganese, 18,000 mg; selenium, 60 mg; zinc, 14,000 mg.

Table 2. The effect of treatments on FI, BW, FCR and PEF of broiler chickens at the end of the first week.

Treatments	FI (g)	BW (g)	FCR	PEF
Control	141.5	94.8	1.492	122.60
T1	136.8	89.0	1.537	118.64
T2	135.3	101.2	1.339	145.49
T3	140.5	97.5	1.447	133.43
T4	140.1	91.5	1.536	121.72
T5	145.6	94.9	1.542	124.47
SEM	3.98	3.49	0.039	6.25

T1: Infected; T2: Infected + Maxus; T3: Infected + Presan; T4: Infected + Clostat; T5: Infected + Presan + Clostat.

Table 3. The effect of treatments on FI, body weight gain (BWG), FCR and PEF of broiler chickens at the end of the second week.

Treatments	FI (g)	BWG (g)	FCR	PEF
Control	389.3	269.6	1.421	201.1
T1	372.4	249.5	1.562	175.5
T2	375.4	274.2	1.358	210.8
T3	380.3	266.1	1.412	205.5
T4	380.1	264.2	1.444	197.5
T5	397.2	256.2	1.499	184.6
SEM	12.65	9.31	0.058	10.69

T1: Infected; T2: Infected + Maxus; T3: Infected + Presan; T4: Infected + Clostat; T5: Infected + Presan + Clostat.

Table 4. Effect of treatments on blood biochemical parameters and liver enzymes of broiler chickens at the end of the first week given experimental diets.

Treatment	Glucose (mg/dl)	Protein (g/dl)	Albumin (g/dl)	Globulin (g/dl)	Triglyceride (mg/dl)	ALT (U/l)	AST (U/l)
Control	239.4	3.09	1.60^c	1.5	99.4	7.22	180.8^b
T1	252.2	2.92	1.62^c	1.3	106.2	8.48	160.5^b
T2	262.6	2.78	2.03^ab	0.8	110.5	8.00	177.2^b
T3	268.2	2.84	1.89^abc	1.0	106.5	8.48	216.2^b
T4	270.2	2.35	1.74^bc	0.6	121.8	6.34	189.1^b
T5	267.0	3.42	2.04^a	1.4	105.3	8.66	280.8^a
SEM	21.40	0.24	0.10	0.25	0.10	1.00	14.46

Different superscript letters (a–c) in a column differ significantly (P < .05).

T1: Infected; T2: Infected + Maxus; T3: Infected + Presan; T4: Infected + Clostat; T5: Infected + Presan + Clostat.

Table 5. Effect of treatment on blood parameters and liver enzymes of broiler chickens at the end of the second week given experimental diets.

Treatment	Glucose (mg/dl)	Total protein (g/dl)	Albumin (g/dl)	Globulin (g/dl)	Triglyceride (mg/dl)	ALT (U/l)	AST (U/l)
Control	275.6	1.56^bc	2.93	1.37	117.9^c	9.84	231.1
T1	282.0	1.31^c	2.52	1.22	127.4^bc	8.92	185.6
T2	294.4	1.67^ab	2.74	1.07	146.2^ab	9.10	186.6
T3	269.2	1.55^bc	2.74	1.18	110.4^c	9.78	225.7
T4	277.0	1.90^a	2.95	1.04	152.8^ab	8.18	229.6
T5	240.2	1.56^bc	2.93	1.03	157.2^a	8.62	182.6
SEM	14.74	1.04	8.78	0.177	0.105	1.04	22.79

Different superscript letters (a–c) in a column differ significantly (P < .05).

T1: Infected; T2: Infected + Maxus; T3: Infected + Presan; T4: Infected + Clostat; T5: Infected + Presan + Clostat.

Figure 1. Mean TAC at the end of the second week, and TBARS concentration in blood of control and treated groups during the first and second weeks of the experiment. Different superscripts in a column differ significantly (P < .05); T1: Infected; T2: Infected + Maxus; T3: Infected + Presan; T4: Infected + Clostat; T5: Infected + Presan + Clostat.