Evaluation of three mycotoxin binders to prevent the adverse effects of aflatoxin B₁ in growing broilers

Figures & data

Table 1. Effects of aflatoxin B1 (AFB₁) and toxin binders (TXB) on growth performance of young broiler chicks^d.

Main effects	ADFI, g	ADG, g	FCR
AFB1, µg/g
0	41.4^a	36.8^a	1.12^b
2	37.3^b	27.4^b	1.14^b
4	34.4^b	23.7^b	1.48^a
TXB
0	37.5	26.8^b	1.49^a
Mycosorb	36.4	31.3^a	1.17^c
Formycin	37.7	28.6^ab	1.34^b
Anzymit	39.4	30.6^a	1.31^b
SEM	2.1	2.3	0.02
P-value
AFB1	<0.001	<0.001	<0.001
TXB	0.108	<0.001	<0.001
AFB1 × TXB	0.332	0.412	0.175

Note: ADG = average daily gain, ADFI = average daily feed intake, FCR = feed conversion ratio.

^a–cMeans values within columns with no common superscripts are significantly different (P < 0.05).

^dData are means for 5 replicates of 10 chickens per group.

Table 2. Effects of aflatoxin B1 (AFB₁) and toxin binders (TXB) on relative organs weight(% of BW) in young broiler chicks^c.

Main effects	Heart	Gizzard	Liver
AFB1, µg/g
0	0.24^b	2.15^b	2.16^b
2	0.31^a	2.52^a	2.50^a
4	0.38^a	2.78^a	2.87^a
TXB
0	0.32	2.59	2.54
Mycosorb	0.30	2.49	2.55
Formycin	0.33	2.58	2.43
Anzymit	0.29	2.57	2.52
SEM	0.021	0.183	0.246
P-value
AFB1	0.016	<0.001	<0.001
TXB	0.085	0.119	0.077
AFB1 × TXB	0.115	0.203	0.142

^a–bMeans values within columns with no common superscripts are significantly different (P < 0.05).

^cData are means for 5 replicates of 2 chickens per group.

Table 3. Effects of aflatoxin B1 (AFB₁) and toxin binders (TXB) on hematological values (%) in young broiler chicks^c.

Main effects	Hematocrit	Eosinophil	Monocyte	Lymphocyte
AFB1, µg/g
0	41.66^a	3.32^a	2.25^a	79.17^a
2	31.34^b	1.92^b	1.73^b	63.16^b
4	32.71^b	2.05^b	1.52^b	62.32^b
TXB
0	31.82^b	1.51^b	1.43^b	66.77^b
Mycosorb	40.77^a	2.89^a	2.11^a	79.77^a
Formycin	40.32^a	2.54^a	1.67^b	78.22^a
Anzymit	39.66^a	2.78^a	2.11^a	74.77^a
SEM	5.42	0.64	0.18	5.46
P-value
AFB1	<0.001	<0.001	0.005	<0.001
TXB	0.004	<0.001	0.003	0.007
AFB1 × TXB	0.062	0.113	0.085	0.057

^a–bMeans values within columns with no common superscripts are significantly different (P < 0.05)

^cData are means for 5 replicates of 2 chickens per group.

Table 4. Effects of aflatoxin B1 (AFB₁) and toxin binders (TXB) on blood metabolites of young broiler chicks^c.

Main effects	GLO	ALB	TP	CHO	LDL	HDL
	(g/dL)			(mg/dL)
AFB1, µg/g
0	2.48^a	1.86^a	4.34^a	62.00	37.14	80.15
2	2.21^b	1.80^b	4.01^b	56.96	35.82	77.24
4	2.25^b	1.79^b	3.94^b	56.72	34.50	73.15
TXB
0	2.23^b	1.88^b	4.11^b	50.06	29.20	70.78
Mycosorb	2.45^a	1.93^a	4.38^a	62.94	38.99	77.53
Formycin	2.53^a	1.92^a	4.45^a	64.54	36.76	82.88
Anzymit	2.48^a	1.94^a	4.42^a	60.69	38.34	76.20
SEM	3.51	2.45	0.14	2.85	2.11	3.58
P-value
AFB1	0.023	0.009	0.018	0.412	0.229	0.329
TXB	0.016	0.024	0.025	0.137	0.526	0.117
AFB1 × TXB	0.157	0.276	0.078	0.250	0.118	0.075

Note: GLO = globulin, ALB = albumin, TP = total protein, CHO = total cholesterol, LDL = low-density lipoprotein, HDL = high-density lipoprotein.

^a-bMeans values within columns with no common superscripts are significantly different (P < 0.05).

^cData are means for 5 replicates of 2 chickens per group.

Table 5. Effects of aflatoxin B1 (AFB₁) and toxin binders (TXB) on minerals concentration (mg/dL) and enzyme activities (U/L) in serum of young broiler chicks^c.

Main effects	Mineral		Enzyme activity
	Ca	P	AST	ALT
AFB1, µg/g
0	10.80^a	8.34^a	189^b	8.00
2	8.51^b	6.25^b	204^a	7.25
4	8.05^b	5.95^b	225^a	8.75
TXB
0	8.30	5.60	219^a	10.22^a
Mycosorb	9.95	7.49	184^b	7.00^b
Formycin	8.84	6.90	223^a	6.67^b
Anzymit	9.38	7.39	196^b	8.11^b
SEM	0.74	1.45	7.3	1.52
P-value
AFB1	0.018	0.015	0.025	0.195
TXB	0.315	0.423	0.014	0.023
AFB1 × TXB	0.276	0.178	0.168	0.416

Note: Ca = calcium, P = phosphorus, AST = aspartate aminotransferase, ALT = alanine aminotransferase.

^a–bMeans values within columns with no common superscripts are significantly different (P < 0.05).

^cData are means for 5 replicates of 2 chickens per group.