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Articles

Minthostachys verticillata essential oils as potential phytogenic additives and chemoprotective strategy on aflatoxin B1 toxicity

ORCID Icon, , , , , & show all
Pages 217-222 | Received 23 Aug 2018, Accepted 25 Apr 2019, Published online: 09 May 2019

Figures & data

Figure 1. Viability of Vero cells exposed to different concentrations of aflatoxin B1 (0–30 µg/mL) for 48 h determined by NRU assays. The results are presented as percentage (mean ± SD) of three independent experiments.

Figure 2. Viability of Vero cells exposed to aflatoxin B1 (10 µg/mL) and different concentrations of M. verticillata essential oil (0–500 µg/mL) for 48 h determined by NRU assays. The results are presented as percentage (mean ± SD) of three independent experiments. *P < .05 and **P < .01 indicates a significant difference compared with the control group (One-way ANOVA test).

Table 1. Micronucleated erythrocytes in the bone marrow cells of male Wistar rat fed aflatoxin B1-contaminated diet (100 mg/kg) and treated with M. verticillata essential oil (0.04%).

Figure 3. Histological examination of internal organs of male Wistar rats fed aflatoxin B1-contaminated diet (100 mg/kg) and treated with M. verticillata essential oil (0.04%) for 45 d. Representative sections of liver, kidney (×400) and intestine (×200).

Figure 4. Effects of administration aflatoxin B1-contaminated diet (100 mg/kg) and M. verticillata essential oil (0.04%) on intestinal villus height of male Wistar rats through 45 d. All data are mean ± SEM of 5 rats/group. *P < .05 (Dunnett’s Multiple Comparison Test).

Table 2. Effect of M. verticillata essential oil on male Wistar rat performance with an AFB1-contaminated diet.