Effects of dietary supplementation with Bacillus licheniformis derived-protease on growth performance, nutrient digestibility and fecal microbial shedding in post-weaned growing pigs

Figures & data

Table 1. Ingredient composition of experimental diets as-fed basis.

Ingredients, %	Post-weaned pigs			Growing pigs
	Phase 1 (wk 0–1)	Phase 2 (wk 2–3)	Phase 3 (wk 4–6)	Phase 4 (wk 7–11)
Extruded corn	47.90	55.93	63.36	–
Yellow corn	–	–	–	48.29
Wheat 11%	–	–	–	14.00
Dehulled soybean meal	18.00	24.00	28.40	–
Fermented soybean meal	8.00	5.00	–	–
Soybean meal 44% (imported)	–	–	–	16.60
Soybean meal 45.4% (local)	–	–	–	10.00
LT Fish meal	2.70	–	–	–
Soy oil	3.20	3.25	3.65	–
Animal fat	–	–	–	4.50
Di-calcium phosphate (DCP)	1.34	1.63	1.36	1.25
Limestone	0.74	0.82	–	0.9
Sugar	2.00	2.00	2.00	–
Whey protein	8.00	3.00	–	–
Lactose	6.70	3.00	–	–
Molasses	–	–	–	3.20
Salt	–	–	–	0.30
L-Lysine-HCL 51%	0.46	0.48	0.38	0.38
DL-Metionine 50%	0.17	0.19	0.16	0.05
L-Threonine 98.5%	0.29	0.20	0.19	0.14
L-Tryptophan 10%	–	–	–	0.01
Choline 50%	0.10	0.10	0.10	0.08
Vitamin premix^a	0.20	0.20	0.20	0.15
Mineral premix^b	0.20	0.20	0.20	0.15
Total	100.00	100.00	100.00	100.00
Calculated composition
Crude protein, %	19.0	18.5	18.0	17.6
Ether extract, %	4.80	4.20	4.40	6.87
Calcium, %	0.75	0.75	0.70	0.75
Total phosphorus, %	0.65	0.65	0.60	0.65
DE, kcal/kg	3,900	3,800	3,700	3563
Lysine, %	1.50	1.40	1.30	1.00
Methionine, %	0.45	0.42	0.39	0.28
Lactose, %	12	5	0	–

^aProvided per kg of complete diet (post-weaned pigs): 11,025 IU vitamin A; 1,103 IU vitamin D₃; 44 IU vitamin E; 4.4 mg vitamin K; 8.3 mg riboflavin; 50 mg niacin; 4 mg thiamine; 29 mg D-pantothenic; 166 mg choline; 33 μg vitamin B₁₂; provided per kg of complete diet (growing pigs): 4,000 IU vitamin A; 800 IU vitamin D₃; 154 mg vitamin E; 2 mg vitamin K₃; 4 mg riboflavin; 20 mg niacin; 4 mg thiamine; 11 mg D-pantothenic; 166 mg choline; 16 μg vitamin B₁₂.

^bProvided per kg of complete diet (post-weaned and growing pigs): 12 mg Cu (as CuSO₄·5H₂O); 85 mg Zn (as ZnSO₄); 8 mg Mn (as MnO₂); 0.28 mg I (as KI); 0.15 mg Se (as Na₂SeO₃·5H₂O).

Table 2. Evaluation of dietary supplementation of protease on growth performance in post-weaned and growing pigs¹.

Items	CON	PROT	P-value
Body weight, kg
Initial	6.61 ± 1.73	6.61 ± 1.73	0.998
Phase 1	8.23 ± 2.02	8.27 ± 1.99	0.969
Phase 2	14.61 ± 3.13	14.92 ± 2.73	0.829
Phase 3	25.94 ± 3.27	27.44 ± 2.94	0.319
Phase 4	50.61 ± 5.77	53.66 ± 5.04	0.250
Phase 1 (wk 0–1)
ADG, g	232.0 ± 59.3	237.8 ± 64.4	0.846
ADFI, g	268.7 ± 63.7	265.6 ± 60.6	0.917
G:F	0.860 ± 0.059	0.889 ± 0.069	0.350
Phase 2 (wk 1–3)
ADG, g	455.9 ± 95.6	474.8 ± 64.7	0.631
ADFI, g	575.8 ± 89.9	616.3 ± 47.2	0.248
G:F	0.786 ± 0.061	0.768 ± 0.058	0.541
Phase 3 (wk 3–6)
ADG, g	539.1 ± 15.7	596.2 ± 48.4	0.008
ADFI, g	846.3 ± 36.8	855.3 ± 48.6	0.664
G:F	0.638 ± 0.031	0.697 ± 0.033	0.001
Phase 4 (wk 6–11)
ADG, g	685.2 ± 76.2	716.3 ± 71.4	0.385
ADFI, g	1489.9 ± 50.8	1505.7 ± 38.8	0.470
G:F	0.460 ± 0.043	0.476 ± 0.049	0.464
Overall
ADG, g	564.2 ± 52.9	597.7 ± 41.7	0.156
ADFI, g	795.1 ± 53.6	810.9 ± 37.0	0.478
G:F	0.709 ± 0.039	0.737 ± 0.040	0.148

Results were presented as mean ± standard deviation.

¹Abbreviation: CON, basal diet; PROT, basal diet +0.5 g/kg protease.

^a,bMeans within the same row with different superscripts differ (P < 0.05).

Table 3. Effect of dietary supplementation of protease on apparent total tract nutrient digestibility in post-weaned and growing pigs¹.

Items, %	CON	PROT	P-value
wk6
Dry Matter	82.83 ± 2.01	85.14 ± 1.94	0.035
Crude protein	80.92 ± 2.11	83.89 ± 2.02	0.012
Gross energy	81.75 ± 2.34	83.99 ± 2.07	0.063
Finish
Dry Matter	80.67 ± 1.29	83.03 ± 1.40	0.004
Crude protein	79.99 ± 0.99	82.02 ± 1.59	0.009
Gross energy	79.93 ± 1.10	80.87 ± 1.79	0.225

Results were presented as mean ± standard deviation.

¹Abbreviation: CON, basal diet; PROT, basal diet +0.5 g/kg protease.

^a,bMeans within the same row with different superscripts differ (P < 0.05).

Table 4. Effect of dietary supplementation of protease on fecal microbial shedding in post-weaned and growing pigs¹.

Items, log10cfu/g	CON	PROT	P-value
wk6
Lactobacillus	7.50 ± 0.18	7.69 ± 0.08	0.109
E. coil	5.52 ± 0.17	5.43 ± 0.13	0.430
Finish
Lactobacillus	7.51 ± 0.22	7.73 ± 0.10	0.122
E. coil	5.68 ± 0.14	5.55 ± 0.17	0.292

Results were presented as mean ± standard deviation.

¹Abbreviation: CON, basal diet; PROT, basal diet +0.5 g/kg protease.

^a,bMeans within the same row with different superscripts differ (P < 0.05).