Effect of cysteine, insulin-like growth factor-1 and epidermis growth factor during in vitro oocyte maturation and in vitro culture of yak-cattle crossbred embryos

Figures & data

Table 1. Effects of combined cysteine, IGF-1 and EGF on yak oocyte maturation and embryo development.

		No. (%) of
	No. of COCs	Matured	Cleaved	Blastocysts
Cys	238	178 (75.20 ± 0.08)^a	168 (70.86 ± 0.12)^a,b	51 (30.42 ± 0.08)^a
IGF-1	233	172 (73.88 ± 0.06)^a	145 (62.23 ± 0.07)^b	44 (30.06 ± 0.05)^a
EGF	233	173 (74.25 ± 0.10)^a	171 (73.03 ± 0.11)^a,c	52 (30.39 ± 0.04)^a
IGF-1 + EGF	253	193 (76.44 ± 0.08)^a	198 (78.32 ± 0.10)^c,d	65 (32.71 ± 0.03)^a
IGF-1 + Cys	247	187 (75.75 ± 0.07)^a	168 (68.25 ± 0.11)^a,b	51 (30.13 ± 0.04)^a
EGF + Cys	255	200 (78.75 ± 0.05)^a,b	182 (71.61 ± 0.08)^a	59 (32.38 ± 0.07)^a
IGF-1 + EGF + Cys	249	210 (84.44 ± 0.02)^b	200 (80.45 ± 0.12)^d	77 (38.67 ± 0.06)^b
Control	250	166 (66.50 ± 0.04) ^c	162 (64.77 ± 0.10)^b	34 (21.16 ± 0.08)^c

Means ± SEM presented. Five replicated trials were carried out, and blastocysts were harvested on Day 7 (Day 0: day of insemination). Blastocyst formation rate was calculated from the cleaved zygotes. ^a,b,c,dMeans in a column with superscript are significantly different (p < .05).