The impact of chronic diarrhoea in Mycobacterium avium subsp. paratuberculosis seropositive dairy cows on serum protein fractions and selected acute phase proteins

Figures & data

Figure 1. (a,b) Representative electrophoretograms in cows: (a) healthy cow, (b) MAP-positive cow with chronic diarrhoea.

Table 1. Differences in the relative concentrations of serum protein fractions (%) and albumin/globulin ratio (A/G) between cows with chronic diarrhoea and clinically healthy cows (mean ± SD).

Variables	Groups of cows		P-value
	Chronic diarrhoea (n = 44)	Healthy (n = 19)
Albumin	33.7 ± 8.0	49.2 ± 4.7	<0.001
α1-globulins	8.5 ± 2.2	5.4 ± 0.6	<0.001
α2-globulins	12.3 ± 2.8	11.5 ± 1.2	n.s.
β1-globulins	8.9 ± 1.4	7.4 ± 0.7	<0.001
β2-globulins	8.4 ± 3.1	7.0 ± 1.4	n.s.
γ-globulins	27.8 ± 4.2	19.4 ± 3.3	<0.001
A/G	0.53 ± 0.18	0.98 ± 0.18	<0.001

Note: P-value – significance of the unpaired t-test, n.s. – not significant, A/G – albumin/globulin ratio.

Table 2. Differences in the concentrations of total serum proteins (TP, g/l) and absolute values of protein fractions (g/l) between cows with chronic diarrhoea and clinically healthy cows (mean ± SD).

Variables	Groups of cows		P-value
	Chronic diarrhoea (n = 44)	Healthy (n = 19)
TP	63.6 ± 10.1	75.5 ± 3.1	<0.001
Albumin	21.7 ± 6.7	37.1 ± 2.8	<0.001
α1-globulins	5.3 ± 1.2	4.1 ± 0.4	<0.001
α2-globulins	7.9 ± 1.3	8.7 ± 1.0	<0.05
β1-globulins	5.6 ± 1.2	5.6 ± 0.6	n.s.
β2-globulins	5.3 ± 1.9	5.3 ± 1.2	n.s.
γ-globulins	17.8 ± 4.2	14.7 ± 2.9	<0.01

Note: P-value – significance of the unpaired t-test, n.s. – not significant, TP – total proteins.

Table 3. Differences in the concentrations of SAA, Hp and CRP between cows with chronic diarrhoea and clinically healthy cows (mean ± SD).

Variables	Groups of cows		P-value
	Chronic diarrhoea (n = 44)	Healthy (n = 19)
SAA (μg/ml)	27.44 ± 45.51	15.69 ± 11.85	n.s.
Hp (mg/ml)	0.238 ± 0.374	0.080 ± 0.020	n.s.
CRP (μg/ml)	45.45 ± 61.65	150.10 ± 72.80	<0.001

Note: P-value – significance of the unpaired t-test, n.s. – not significant.