Characterization in vitro of new bacterial strains showing potentially probiotic crossed effect against vibriosis in relevant fish species for marine aquaculture

Figures & data

Table 1. Pathogenic strains used in testing antagonistic effect.

Pathogen strains	Reference
Vibrio anguillarum 112P	CECT
Vibrio anguillarum 975–1	CECT
Vibrio anguillarum 507	CECT
Vibrio anguillarum 4347	CECT
Vibrio harveyi 525	CECT
Vibrio alginolyticus 521	CECT

Note: CECT: Spanish type culture collection.

Table 2. In vitro antagonistic effect and identification of potential probiotic strains isolated from in seabass, meagre and common sole against Vibrio.

Pathogen strains tested	Potential probiotic strains (No.)
	1	2	3	4	5	6	7
Vibrio anguillarum 112P	+	+	+
Vibrio anguillarum 975–1	+			+	+
Vibrio anguillarum 507	+	+	+
Vibrio harveyi 525	+	+	+
Vibrio alginolyticus 521	+				+
Vibrio anguillarum 4347	+			+	+	+	+

Notes: Source of pathogens strains tested is showed in Table 1.

1. – Alcaligenes faecalis subsp. faecalis-1

2. – Alcaligenes faecalis subsp. faecalis-2

3. – Pseudomonas viridiflava

4. – Shewanella putrefaciens

5. – Alcaligenes faecalis subsp. faecalis-3

6. – Proteus penneri-1

7. – Proteus penneri-2

+ potential probiotic strain isolated shows antagonistic effect against any pathogen strains tested.

Table 3. Percentage of survival to pH gradients tested for potential probiotic strains isolated from seabass, meagre and common sole.

pH	Probiotic strains tested (No.) % survival†† (mean ± sd)
	1	2	3	4	5	6	7
pH 7	100%	100%	100%	100%	100%	100%	100%
pH 6	91.6 ± 3.3	71.8* ± 2.2	85.7 ± 3.5	81.4 ± 2.5	81.3 ± 8.0	89.5 ± 3.3	74.1* ± 4.4
pH 5	74.3* ± 4.5	41.5* ± 3.5	65.5* ± 1.2	65.4* ± 3.4	62.5* ± 3.2	72.7* ± 2.0	69.6* ± 2.9
pH 4	50.4* ± 10.6	25.2* ± 2.7	55.6* ± 0.8	35.5* ± 2.5	43.6* ± 2.6	66.5* ± 3.5	43.0* ± 2.0
pH 3	6.3* ± 3.8	4.9* ± 1.84	11.5* ± 0.9	11.1* ± 1.3	2.9* ± 0.2	63.5* ± 3.0	18.8* ± 1.8

Notes: 1. – Alcaligenes faecalis subsp. faecalis-1

2. – Alcaligenes faecalis subsp. faecalis-2

3. – Pseudomonas viridiflava

4. – Shewanella putrefaciens

5. – Alcaligenes faecalis subsp. faecalis-3

6. – Proteus penneri-1

7. – Proteus penneri-2

^††percentage of survival for each pH value was carried out in triplicated.

*statistically significant differences were found for the percentage of survival of a pH value with regard to that showed for the same strain at pH 7 (P < 0.05).