Comparative analysis of melanin deposition between Chishui silky fowl and Taihe silky fowl

Figures & data

Table 1. Primers used for quantitative real-time PCR.

Primer	Sequences (5’–3’)	GenBank accession number	Product Length (bp)
GAPDH	Forward: GGGTAGTGAAGGCTGCTGCTGAT Reverse: CCGCATCAAAGGTGGAGGAAT	XM-005016745.1	114
ASIP	Forward: AACCAAGGCCCACACCTGC Reverse: CGGCATTTGCACAACGCACAG	JF770438.1	100
TYRP1	Forward: CAGAAGCTCAGTTCCCTCG Reverse: CCATACGCTTCTTCAACCA	NM-205045.2	226
TYR	Forward: CACTCTTAGGTGGCTCCAATGTG Reverse: CAGTCCCAGTAGGGGATGGTGAA	NM-204160.1	156
MC1R	Forward: TCCGTCGTGTCCTCCCTCT Reverse: CCAGCGCGAACATGTGAA	NM-00103146.1	262
MITF	Forward: GGTCAACAGGGTCATCAAACAAGAA Reverse: CCGAGGTTGTCACTGAAGGTGAT	NM-205029.1	135

Figure 1. Comparative analysis of lightness values in the Taihe silky fowl and Chishui silky fowl. Values are represented as mean ± SD (n = 30, 30 birds per breed and 15 per gender). ^a–bColumns with different superscript letters are significantly different between breeds (P < 0.05).

Table 2 . Melanin content of different tissues in the Taihe silky fowl and Chishui silky fowl.

	Tissue	Eumelanin content (μg/mg)	Phaeomelanin content (μg/mg)
Taihe Rooster	Chest muscle	271.20 ± 95.52	1102.38 ± 354.35
Taihe Hen		280.21 ± 91.08	1139.31 ± 349.01
Chishui Rooster		276.02 ± 146.99	1121.86 ± 553.67
Chishui Hen		251.95 ± 146.36	1015.91 ± 545.05
Taihe Rooster	Leg muscle	483.39 ± 183.02^a	1755.51 ± 675.88^a
Taihe Hen		543.93 ± 348.64^b	2162.71 ± 1310.53^b
Chishui Rooster		502.74 ± 248.68^ab	1997.51 ± 931.65^ab
Chishui Hen		335.10 ± 221.18^a	1355.92 ± 844.16^a
Taihe Rooster	Skin	511.80 ± 126.81	2079.77 ± 496.46
Taihe Hen		614.10 ± 214.77	2471.23 ± 830.24
Chishui Rooster		543.43 ± 168.11	2207.13 ± 662.91
Chishui Hen		518.88 ± 168.61	2150.01 ± 697.86
Taihe Rooster	Liver	580.86 ± 354.82	2387.35 ± 1393.79
Taihe Hen		489.89 ± 243.56	2029.94 ± 933.24
Chishui Rooster		498.08 ± 278.27	2029.27 ± 1097.37
Chishui Hen		412.08 ± 172.21	1694.10 ± 657.92

Values are represented as mean ± SD (n = 30, 30 birds per breed and 15 per gender). ^a–bColumns with different superscript letters are significantly different between breeds (P < 0.05).

Figure 2. The mRNA expression levels of ASIP, MC1R, MITF, TYRP1 and TYR in the four tissues of Taihe silky fowl and Chishui silky fowl. ^a-dColumns with different superscript letters are significantly different between breeds (P < 0.05). Values are represented as mean ± SD (n = 6–8). a: ASIP mRNA expression level in Taihe silky fowl and Chishui silky fowl; b: MC1R mRNA expression level in Taihe silky fowl and Chishui silky fowl; c: MITF mRNA expression level in Taihe silky fowl and Chishui silky fowl; d: TYRP1 mRNA expression level in Taihe silky fowl and Chishui silky fowl; e: TYR mRNA expression level in Taihe silky fowl and Chishui silky fowl.