Analysis of intestinal bacterial diversity and its gene function prediction in black-capped capuchin (Sapajus apella)

Figures & data

Figure 1. Schematic diagram of fecal sample collection sites of black-capped capuchins; The triangle marker is the fecal sample collection point.

Table 1. Species composition of bacteria at different taxonomic levels.

Taxonomic level	ES (exclusive)	Two groups (shared)	HT (exclusive)
ASV	1345	36	915
Genus	50	102	58
Family	24	56	19
Phylum	2	11	3

Table 2. Analysis summary of α diversity index at different classification levels.

Diversity Index	Classification level	ES	HT	P
Chao	ASV	778.67 ± 126.89	527.45 ± 128.85	0.074
	Genus	122.38 ± 10.04	126.54 ± 17.58	0.740
	Family	66.00 ± 5.00	63.00 ± 7.00	0.578
	Phylum	13.00 ± 0.00	13.67 ± 0.58	0.116
Shannon	ASV	5.99 ± 0.22	4.82 ± 0.60	0.034
	Genus	3.59 ± 0.09	3.04 ± 0.28	0.032
	Family	2.95 ± 0.07	2.47 ± 0.24	0.028
	Phylum	1.23 ± 0.09	1.01 ± 0.05	0.022
Shannon even	ASV	0.90 ± 0.02	0.77 ± 0.07	0.032
	Genus	0.75 ± 0.01	0.63 ± 0.04	0.009
	Family	0.70 ± 0.01	0.60 ± 0.04	0.012
	Phylum	0.48 ± 0.04	0.39 ± 0.02	0.015

Figure 2. Cluster analysis of intestinal bacterial flora of black-capped capuchins in different regions.

Figure 3. Circos plot of the composition of the intestinal bacterial community at different taxonomic levels; A, Phylum; B, Family; C, Genus.

Figure 4. Analysis of differences and significance tests between species groups at different taxonomic levels; *P < 0.05 and **P < 0.01.

Figure 5. Functional classification statistics and difference test of metabolic genes in intestinal bacteria; A, COG functional abundance statistics; B, COG functional abundance Student’s t-test; *P < 0.05 and **P < 0.01.

Data Availability Statement

Genetic sequencing data (BioSample SAMN26816508, SAMN26816509, SAMN26816510, SAMN26816511, SAMN26816512, SAMN26816513) of six fecal samples from black-capped capuchin completed by Shanghai Majorbio Bio-Pharm Technology Co., Ltd, and this study is available in GenBank under BioProject number PRJNA818119.