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Research Article

Comparative study of leucine on digestive enzyme secretion, ISR and mTOR pathway in cattle and porcine pancreas in vitro

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Article: 2335942 | Received 26 Sep 2022, Accepted 23 Mar 2024, Published online: 09 Apr 2024

Figures & data

Table 1. Ingredients and chemical composition of the cattle and pigs (air-dry basis).

Table 2. The PCR primers used to amplify the genes encoding PKB, 4E-BP1, S6K1, eIF-2α, ATF4 and β-actin in pig.

Table 3. The PCR primers used to amplify the genes encoding PKB, 4E-BP1, S6K1, eIF-2α, ATF4 and β-actin in cattle.

Table 4. Interaction of Leu on pancreatic enzyme activity of different animals in vitro.

Figure 1. The effect of Leu on the expression of key genes in the mTOR pathway in the pancreatic tissue of cattle and pigs in vitro.

Figure 1. The effect of Leu on the expression of key genes in the mTOR pathway in the pancreatic tissue of cattle and pigs in vitro.

Figure 2. The effect of Leu on the expression and phosphorylation of key proteins in the mTOR signaling pathway in the pancreatic tissue of cattle and pigs in vitro.

Figure 2. The effect of Leu on the expression and phosphorylation of key proteins in the mTOR signaling pathway in the pancreatic tissue of cattle and pigs in vitro.

Figure 3. The effect of Leu on the expression of key genes in the ISR pathway in the pancreatic tissue of cattle and pigs in vitro.

Figure 3. The effect of Leu on the expression of key genes in the ISR pathway in the pancreatic tissue of cattle and pigs in vitro.

Figure 4. The effect of Leu on the expression and phosphorylation of key proteins in the ISR signaling pathway in the pancreatic tissue of cattle and pigs in vitro.

Figure 4. The effect of Leu on the expression and phosphorylation of key proteins in the ISR signaling pathway in the pancreatic tissue of cattle and pigs in vitro.

Data availability statement

The data used to support the findings of this study are available from the corresponding author upon request.