Interindividual variation of malate dehydrogenase activity in the oyster Crassostrea virginica

Abstract

Intertidal environments are dynamic, stressful niches and variation in physiological parameters may determine distribution and survival of individuals in a population. We demonstrated that mitochondria of the oyster Crassostrea virginica oxidize malate more readily than other Krebs cycle intermediates and investigated the level of interindividual variability in oyster malate dehydrogenase (MDH) activity and total protein content in muscle tissues. Both MDH activity and total protein evidenced a high level of interindividual variation in heart and adductor among a sample of more than 50 oysters. Normalization to total DNA failed to explain the variation in either MDH activity or protein content of phasic adductor and explained less than 40% of the variation in heart. This range of MDH titers defines a continuum of biochemical phenotypes important for understanding the relative selection forces operative on metabolic pathways within the muscles of the oyster.

Keywords:

• Interindividual variation
• phenotype
• natural selection
• oyster
• Crassostrea
• malate dehydrogenase
• protein
• heart
• adductor

Acknowledgements

We are grateful for the assistance that T. Szela, L. Kendall, and C. Hoover provided with tissue preparations. This research was supported by grants to AGM from NSF OCE-0095459 and the University of Delaware Research Foundation.