Angiopoietins in the bone marrow microenvironment of acute lymphoblastic leukemia

Figures & data

Figure 1 (A) MSCs (P2) derived from bone marrow of acute lymphoblastic leukemia. (B) Adipogenic differentiation of MSCs (P2) derived from bone marrow of acute lymphoblastic leukemia (oil red O staining) (MSCc and adipogenic differentiation are shown in 10× magnification).

Table 1 Demographic data of patients and controls are demontrated. (A) The age and gender of the patients and controls are stated. (B) Differential blood count, BM blast percentage at diagnosis, ALL subtypes, chromosome abnormalities, risk groups and remission status (after induction therapy) of patients are stated. Two patients had t(11q23) which was unfavorable and two had t(12;21) which was favorable

	Patients	Control group
(A)
Age	Mean ± SD	7.4 ± 5.8
	Min–max	1.1−17.0
Gender	Female	6 (30%)
	Male	14 (70%)
	Total	20 (100%)
(B)
Differential blood count	Mean ± SD
Hemoglobin (g/dl)	8.7 ± 3.1
WBC (1/mm^3)	27 030 ± 33 859
Absolute blast count (1/mm^3)	20 819 ± 29 238
Platelet (1/mm^3)	66 150 ± 64 706
BM blast percentage at diagnosis	90.7 ± 9.3
ALL subtypes, n
B cell lineage	17
T cell lineage	3
Chromosome abnormalities, n
Favorable	2
Unfavorable	2
No abnormaly	16
Risk groups, n
Low risk	7
Intermediate risk	8
High risk	5
Remission status after induction, n
Remission	18
Not remission	2

Table 2 Cytokine levels of patients at time of diagnosis and after induction therapy (on day +33) and control groups. The cytokine levels were measured both in BM plasma and MSCs supernatant

	Ang 1 (pg/ml)	Ang 2 (pg/ml)	Ang 1/Ang 2 ratio	SDF 1α (pg/ml) ml)
	Median (range)	Median (range)		Median (range)
BM plasma
At diagnosis (n = 20)	87.09	1371.78	0.07	29.50
	(2414.59)	(1502.95)		(37.39)
On day +33 (n = 20)	2002.40	1018.57	1.95	40.81
	(11820.57)	(1179.87)		(200.09)
P value*	0.002	0.03	0.001	0.006
Control group (n = 8)	971.29	754.94	1.25	41.09
	(1520.52)	(236.5)		(28.68)
P value**	<0.0001	<0.0001	<0.0001	0.023
Supernatant
At diagnosis (n = 20)	5963.45	1020.23	7.6	87.47
	(40383.29)	(1557.95)		(197.12)
On day +33 (n = 20)	10315.00	1044.13	9.0	120.92
	(23437.74)	(1732.83)		(200.09)
P value*	>0.05	>0.05	>0.05	>0.05
Control group (n = 8)	13788.70	958.14	18.7	38.50
	(24147.30)	(1560.77)		(155.78)
P value**	>0.05	>0.05	0.035	>0.05

*Comparison of patients at time of diagnosis versus on day +33 (after induction therapy).

**Comparison of patients at time of diagnosis versus control group.