The role of rs1984112_G at CD36 gene in increasing reticulocyte level among sickle cell disease patients

Figures & data

Table 1. Demographic and biological data (steady state) of studied population.

	SS patients (63)	Sβ° patients (36)	p
Age (mean)	12 ± 6	10 ± 5	0.425
Sex ratio (M/F)	36/27	20/16	0.423
Hb (g/dl)	8.3 ± 0.7	11 ± 1.02	0.106
RBC (1012/l)	2.89 ± 1.02	3.69 ± 1.2	0.425
MCV (fl)	77.2 ± 1.3	70 ± 2.7	0.059
MCH (pg)	35.7 ± 1.02	37 ± 2	0.132
HbA	0	0	1
HbS (%)	86.4 ± 0.4	85 ± 2.1	0.155
HbF (%)	10.6 ± 4.3	11 ± 6.02	0.245
HbA2	3 ± 0.1	5 ± 0.9	0.063
Reticulocyte (%)	9.8571 ± 4.89	9.0833 ± 4.783	1
BT (µM)	34.92 ± 17.33	36.76 ± 27.77	1
LDH (UI/l)	430.92 ± 177.34	381.61 ± 140.38	0.856

SS: homozygous of β globin gene mutation.

The demographic and hematologic values are indicated as mean ± standard deviation.

Hb: hemoglobin; RBC: red blood cell; MCV: mean corpuscular volume; MCH: mean corpuscular hemoglobin; RDW: red blood distribution width; BT: total bilirubin; LDH: lactate dehydrogenase.

Statistics for the comparison of demographic and hematological variables between the two groups were performed using the t-test and chi-square test as appropriate (SPSS 16.0).

Table 2. Association of rs1982112 with hemolysis parameters.

rs1982112	n	Mean of reticulocyte (%)	BT (µM)	LDH (UI/l)	P1	P2	P3
AA	40	8.20 ± 3.87	35.56 ± 21.40	412.99 ± 165.84	1*	1*	1*
AG	47	10.23 ± 4.15	40.20 ± 20.50	399.16 ± 155.34	0.022	0.660	0.433
GG	12	14.63 ± 7.04	41.15 ± 11.03	410.22 ± 135.56	0.000	0.520	0.416

Reticulocyte level is indicated as mean ± standard deviation.

BT: total bilirubin; LDH: lactate dehydrogenase.

p: index of significance; each value <0.05 is considered as significant, P1: association with reticulocyte, P2: association with BT, and P3: association with LDH.

Table 3. Genotypic and allelic repartition of the rs1984112 among patients according to the severity of the disease.

rs1984112	SCA (severe) n = 86	SCA (not severe) n = 13	p
AA	34	6	1*
AG	42	5	0.775
GG	10	2	1
A	0.64	0.65	1*
G	0.36	0.35	1

p: index of significance; each value <0.05 is considered as significant.

1*: reference group.

Table 4. Genotypic and allelic repartition of the rs1984112 among patients according to each complication.

	VOC			Osteonecrosis			Stroke			Frequent infection			Priapism			Acute syndrome
rs1984112	−	+	p	−	+	p	−	+	p	−	+	p	−	+	p	−	+	p
AA	8	32	1*	38	2	1*	35	5	1*	28	12	1*	39	1	1*	30	10	1*
AG	8	39	0.721	41	6	0.279	44	3	0.462	32	15	1	45	2	1	38	9	0.691
GG	6	6	0.051	9	3	0.074	11	1	1	10	2	0.475	11	1	0.412	10	2	0.709
A	0.545	0.66	1*	0.66	0.45	1*	0.63	0.72	1*	0.63	0.67	1*	0.65	0.5	1*	0.63	0.69	1*
G	0.45	0.33	0.240	0.34	0.55	0.089	0.37	0.28	0.608	0.37	0.33	0.885	0.35	0.5	0.461	0.37	0.31	0.372

p: index of significance; each value <0.05 is considered as significant.

1*: reference group.

(−): absence; (+): presence.

VOC: vaso-occlusive crisis