Prognostic impact of CD200 and CD56 expression in adult acute lymphoblastic leukemia patients

Figures & data

Figure 1. Shows the expression of CD200 and CD56 in adult B-ALL case. The patient is positive for CD200 but negative for CD56.

Table 1. Hematological and clinical data in CD200⁺ B-ALL patients vs. CD200⁻ B-ALL patients.

Hematological and clinical data	Adult B-ALL (n = 70)		p
	CD200^− (n = 23)	CD200^+ (n = 47)
Age (years) median (range)	18–50	24–52	>0.05
Sex
Male (n)	17	23	0.029**
Females (n)	6	24
Pallor (n)	12	24
Fever (n)	16	35	0.863
Weight loss (n)	9	13	0.400
Pain (n)	12	21	0.429
Bleeding tendency (n)	4	12	0.729
Bone ache (n)	5	6	0.373
Splenomegaly (n)	15	40	0.493
Hepatomegaly (n)	21	36	0.031*
CNS infiltration (n)	2	8	0.001
CD34^+ (n)	8	27	0.001
Total leucocytic count (×10^9/l) Mean (range)	23.3 (1.5–520)	26.6 (1.6–374.8)	0.839
Hemoglobin concentration (g/dl) Mean (range)	8.1 (3.8–12.2)	6.9 (3–13.8)	0.046*
Platelet count (×10^9/l) Mean (range)	92.3 (7–582)	49.9 (5–186)	0.027**
Peripheral blasts%  Mean (range)	45 (0–88)	85 (70–95)	0.053
Marrow blasts%  (range)	88.5 (62–96)	90 (75–98)	0.837
FAB subtypes
L1 (n = 21)	11	10	0.001
L2 (n = 42)	6	36
L3 (n = 7)	6	1
Induction remission response
CR (n)	13	29	>0.05
PR (n)	2	4
Resistant (n)	2	5
Induction death (n)	5	9
Cytogenetics
Favorable (n)	9	18	0.990
Intermediate (n)	9	17
High (n)	5	12
LDH  (U/l) Mean (range)	1105.85 (176–9373)	616.5 (131–3997)	0.080

Table 2. Hematological and bio-chemical data in CD56⁺ B-ALL vs. CD56⁻ ALL patients.

Hematological and clinical data	Adult B-ALL	Adult B-ALL	p
	CD56^− (n = 63)	CD56^+ (n = 7)
Age (years)	18–52	28–50	>0.05
Sex
Male (n)	36	4	0.629
Female (n)	27	3
Pallor (n)	33	4	0.706
Fever (n)	46	3	1
Pain (n)	28	5	0.242
Bleeding tendency (n)	14	2	0.655
Bone ache (n)	9	5	0.302
Splenomegaly (n)	49	2	0.349
Hepatomegaly (n)	60	2	0.334
Lymphadenopathy (n)	49	4	0.067
CNS infiltrations (n)	4	6	0.001
CD34^+ (n)	32	3	0.001
Total leucocytic count (×10^9/l) Mean (range)	26.6 (1.5–520)	18.3 (1.7–113.0)	0.236
Hemoglobin concentration (g/dl) Mean (range)	7.17 (3.0–13.8)	7.7 (6.8–10.6)	0.129
Platelet count (×10^9/l) Mean (range)	51.5 (5–582)	140 (26–397)	0.024*
Peripheral blasts (%) Mean (range)	90 (62–98)	90 (80–90)	0.214
Marrow blasts (%) Mean (range)	70 (0–98)	80 (55–93)	0.201
FAB subtypes
L1 (n = 21)	19	2	0.42
L2 (n = 42)	38	4
L3 (n = 7)	6	1
Cytogenetics
Favorable	23	2	0.45
Intermediate	25	1
High	15	4
Induction remission response
CR	40	1	0.018*
PR	5	1
Resistant	7	2
Induction death	11	3
LDH (U/l) Mean (range)	714.9 (190–9373)	461 (131–7414)	0.564

CR: complete remission; PR: partial remission.

Table 3. Cox regression analysis for prediction of OS in adult B-ALL cases.

		Univariate				Multivariate
		p	HR	95% CI		p	HR	95% CI
Age (years)		.170	1.015	.994	1.036
Sex		.803	.922	.489	1.738
Weight loss		.894	1.046	.537	2.038
Bleeding tendency		.975	.988	.481	2.033
Lymphadenopathy		.430	.754	.374	1.520
Hepatomegaly		.392	1.465	.612	3.509
Splenomegaly		.419	1.379	.633	3.005
Total leucocytic count (×10^9/l)		.858	1.000	.996	1.003
Hemoglobin concentration (g/dL)		.548	.958	.834	1.101
Platelet count (×10^9/l)		.915	1.000	.996	1.003
BM blasts (%)		.022	1.061	1.009	1.116	.369	1.027	.969	1.088
LDH (U/l)		.252	1.023	1.004	1.760
Albumin (g/dl)		.004	.443	.253	.776	.112	.635	.363	1.112
Uric acid (mg/dl)		.436	.970	.899	1.047
CR		<0.001	.146	.072	.296	<0.001	.092	.037	.227
CNS infiltration		.721	.800	.235	2.723
Cytogenetics	Low	.403	1.500	.579	3.885
	Intermediate	.096	.542	.263	1.116
	High	.183	.566	.245	1.307
CD200 (%)		.007	1.014	1.004	1.024	<0.001	1.022	1.010	1.034
CD56 (%)		.035	1.016	1.001	1.031	.023	1.511	1.093	1.930

Table 4. Cox regression analysis for prediction of DFS in adult B-ALL cases.

		Univariate				Multivariate
		p	HR	95% CI		p	HR	95% CI
Age (years)		.103	1.025	.995	1.056
Males		.894	1.059	.452	2.483
Weight loss		.320	1.792	.567	5.658
Bleeding tendency		.159	.229	.030	1.779
Lymphadenopathy		.711	1.283	.344	4.777
Hepatomegaly		.705	1.342	.293	6.151
Splenomegaly		.922	1.068	.288	3.955
Total leucocytic count (×10^9/l)		.159	1.003	.999	1.007
Hemoglobin concentration (g/dl)		.983	.997	.767	1.296
Platelet count (×10^9/l)		.665	1.001	.995	1.007
BM blasts (%)		.086	1.060	.992	1.133
LDH (U/l)		.351	1.025	1.009	1.801
Albumin (g/dl)		.022	.133	.023	.749	.053	.179	.031	1.021
Uric acid (mg/dl)		.984	1.001	.876	1.144
CR		.091	.267	.058	1.233
Cytogenetics	Low	.146	2.651	.713	9.862
	Intermediate	.864	.896	.253	3.167
	High	.317	.432	.083	2.235
CD200 (%)		.003	1.022	1.007	1.037	.039	1.022	1.001	1.043
CD56 (%)		.013	1.022	1.005	1.039	.019	1.036	1.006	1.067

Figure 2. OS in CD200⁺ vs. CD200⁻ B-ALL patients; the OS is shorter in CD200⁺ group as compared to CD200⁻ one (p = 0.042).

Figure 3. DFS in CD200⁺ vs. CD200⁻ B-ALL patients; the DFS is shorter in CD200⁺ group as compared to CD200⁻ one (p = 0.006).

Figure 4. OS in CD56⁺ vs. CD56⁻ B-ALL patients; the OS is shorter in CD56⁺ group as compared to CD56⁻ one (p = 0.003).

Figure 5. DFS in CD56⁺ vs. CD56⁻ B-ALL patients; the DFS is shorter in CD56⁺ group as compared to CD56⁻ one (p = 0.007).