Association of cytotoxic T-lymphocyte antigen 4 gene with immune thrombocytopenia in Chinese Han children

Figures & data

Table 1. PCR primers for each detected SNP.

SNP	5′–3′ primers	Tm	Restriction enzyme
rs11571315	P1: TTAAAAAGTGAAAAACAAATGTTCCTG	58°C	Tas I
	P2: ACTTTAGCCCATGTTATTCTTCTTGT
rs3087243	P1: AGTGCTTGATTGCGTGG	56°C	HpyCH4IV
	P2: TGCTGAGACTATACATTGGTTAAG

Tm, annealing temperature.

Figure 1. CTLA4 linkage blocks constructed by 16 SNPs from 1 the 1000 genomes project database. These SNPs have a MAF ≧0.05 and r² ≧ 0.80.

Table 2. Clinical characteristic of ITP patients and healthy controls.

Items	Cases (n = 102)	Controls (n = 175)	p value
Age (year)	5.43 ± 3.21	6.06 ± 3.77	0.16
Males (%)	30 (29.41)	52 (29.7)	0.95
Platelet (×10^9/l)	11.74 ± 3.96	194.52 ± 27.59	<0.0001
The number of Acute ITP	78	0
The number of Chronic ITP	24	0.
PAIgG, ng/10^7 platelet	94.15 ± 8.2	37.36 ± 3.7	<0.0001

Table 3. Genotypes and alleles frequencies distribution of CTLA4 rs11571315 and rs3087243 in patients with ITP and controls.

SNPs	Genotypes	Cases (%)	Con (%)	Χ2	p	Alleles	Cases (%)	Con (%)	Χ2	p	OR (95%C I)
rs11571315	GG	36(35.29)	68(38.86)	1.03	0.31	G	122(59.80)	224(64.00)	0.97	0.33	ref.
	GA	50(49.02)	88(50.29)			A	82(40.20)	126(36.00)			1.2(0.84-1.7)
	AA	16(15.69)	19(10.86)
rs3087243	GG	51(50.00)	95(54.29)	0.78	0.37	G	146(71.57)	262(74.86)	0.72	0.4	ref.
	GA	44(43.14)	72(41.14)			A	58(28.43)	88(25.14)			1.18(0.57-1.24)
	AA	7(6.86)	8(4.57)

OR, Odds ratios; CI, Confidence interval.

Figure 2. eQTL analysis of CTLA4 gene from the GTEx database. (A) rs11571315; (B) rs3087243.

Figure 3. The mRNA level difference of CTLA4 between SCZ and healthy controls.