Brain region-specific effects of immobilization stress on cholinesterases in mice

Figures & data

Table 1. Primer sequences and protocols used in PCR reactions. The initial denaturation was performed at 95 °C for 3 min. The final polymerization lasting 10 min was performed at 72 °C in all cases.

Gene	Primer sequence	Fragmentsize	Denaturation(°C)/time (s)	Annealing(°C)/time (s)	Polymerization(°C)/time (s)	Numberof cycles	Reference
AChET	5′-GCA GCA ATA TGT GAG CCT GA-3′	197	94/30	60/30	72/2	37	(Kawashima et al., 2007)
	5′-GGT CGA ACT GGT TCT TCC AG-3′
BChE	5′-CCC TGG AGT GAG CAG ATT GG-3′	133	94/30	60/30	72/2	37	This study
	5′-TGC AGG GCA GAT GAT GTT GT-3′
AChER	5′-GGT AGG CGC ATG GAG TGG GGG-3′	166	94/30	60/30	72/2	37	(Perrier et al., 2005)
	5′-GCC GCC TCC CCA TGG GCG GGG-3′
GAPDH	5′-CGT CTT CAC CAC CAT GGA GA-3′	299	94/30	60/30	72/2	37	(Zarghooni et al., 2007)
	5′-CGG CCA TCA CGC CAC AGC TT-3′

Figure 1. Relative changes in mRNA expression after stress in WT and CRH-KO mice expressed as ratio relative to unstressed control. Ordinate shows duration of immobilization, abscissa gives mRNA expression ratio relative to control (ctrl). Control mice (intact mice, time = 0) were not immobilized. If there is significant difference, then the respective increase/decrease is shown; if there is no difference, then the ratio is set to 1.0. Data are expressed as mean ± SEM. One-way ANOVA with SNK post hoc analysis (n = 4 in all groups). Abbreviations in inset key: STR: striatum; AMY: amygdala; CA3: CA3 hippocampal area; PCx: parietal cortex; WT: wild type mice; KO: CRH-KO mice; R: right hemisphere; L: left hemisphere; AChE: acetylcholinesterase; AChE_R: readthrough variant of acetylcholinesterase; BChE: butyrylcholinesterase. For individual changes expressed as measured values see Supplementary Figures 1–5.

Figure 2. Changes in acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) activities in KO mice expressed as ratio relative to unstressed control (ctrl). Control mice (intact mice, time =0) were not immobilized. Ordinate shows duration of immobilization, abscissa gives mRNA expression ratio relative to control. If there is significant difference, then the respective increase/decrease is shown; if there is no difference, then the ratio is set to 1.0. Data are expressed as mean ± SEM. One-way ANOVA with SNK post hoc analysis (n = 4 in all groups). Abbreviations in inset key: STR: striatum; AMY: amygdala; CA3: CA3 hippocampal area; VTA: ventral tegmental area; PCx: parietal cortex; WT: wild type mice; KO: CRH-KO mice; R: right hemisphere; L: left hemisphere; AChE: acetylcholinesterase; BChE: butyrylcholinesterase. For individual changes expressed as measured values see Supplementary Figures 6–9.

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