Acute restraint stress enhances calcium mobilization and proliferative response in splenic lymphocytes from mice

Figures & data

Figure 1 (a) Time course of changes in [Ca^{2 +} ]_i in response to Con A (2 μg/ml) in splenic lymphocytes from control and acute restraint-stressed mice; (b) effects of Con A and Con A plus verapamil on [Ca^{2 +} ]_i in splenic lymphocytes from control (n = 7 per group) and acute restraint-stressed (n = 9 per group) mice. Fura-2-loaded lymphocytes were incubated for 8 min with or without Con A. Verapamil was added 10 min before exposure to Con A. The net Δ[Ca^{2 +} ]_i is the difference in the increase in [Ca^{2 +} ]_i 8 min after addition of Con A or Con A plus verapamil, vs. the vehicle. Data are presented as group means ± SEM. Statistically significant differences from control are indicated as: ^*P < 0.05, ^**P < 0.01.

Figure 2 (a) Time course of changes in [Ca^{2 +} ]_i in response to LPS (25 μg/ml) in splenic lymphocytes from control and acute restraint-stressed mice; (b) effect of LPS on [Ca^{2 +} ]_i in splenic lymphocytes from control (n = 6 per group) and acute restraint-stressed (n = 7 per group) mice. Fura-2-loaded lymphocytes were incubated for 8 min with or without LPS. The net Δ[Ca^{2 +} ]_i is the difference in the increase in [Ca^{2 +} ]_i 8 min after addition of LPS vs. vehicle. Data are presented as group means ± SEM.

Figure 3 Proliferative effects of Con A and Con A plus verapamil on splenic lymphocytes from control (n = 7 per group) and acute restraint-stressed (n = 7 per group) mice. Lymphocytes were stimulated for 42 h with Con A or Con A plus verapamil, and proliferation was measured by MTT assay. Verapamil was added 10 min before exposure to Con A. Data are presented as group means ± SEM. Statistically significant differences vs. control are indicated as: ^*P < 0.05, ^**P < 0.01. OD₅₇₀, optical density at 570 nm.

Figure 4 Proliferative effect of LPS on splenic lymphocytes from control (n = 7 per group) and acute restraint-stressed (n = 7 per group) mice. Lymphocytes were stimulated for 42 h with various concentrations of LPS, and proliferation was measured by MTT assay. Data are presented as group means ± SEM. OD₅₇₀, optical density at 570 nm.