Abstract
Chemical examination of the methanolic extract from the stem bark of Daphne feddei led to the isolation of three new dicoumarin glucosides (1–3), and eight known coumarins, dicoumarins and dicoumarin glucosides. Their structures were elucidated by extensive analysis of spectral data and comparison with the literature values. All compounds were tested for inhibitory activity against lipopolysaccharide-induced NO production in RAW 264.7 macrophages, and compounds 4 and 5 showed potent inhibitory activity with IC50 values of 0.161 and 0.127 μM, respectively.
Acknowledgements
This work was financially supported by program NCET Foundation, NSFC (30725045), the Special Program for New Drug Innovation of the Ministry of Science and Technology, China (2009ZX09311-001, 2008ZX09308-005), Shanghai Leading Academic Discipline Project (B906) and in part by the Scientific Foundation of Shanghai, China (09DZ1975700, 09DZ1971500).