Two new bis-C₂₀-diterpenoid alkaloids with anti-inflammation activity from Aconitum bulleyanum

Abstract

Two new bis-C₂₀-diterpenoid alkaloids, bulleyanines A and B (1 and 2), were isolated from Aconitum bulleyanum. Their structures were elucidated by comprehensive spectroscopic analyses including UV, IR, MS, 1D and 2D NMR. Biological activity tests indicated that compound 1 exhibited significant inhibitory activity against nitric oxide (NO) production in lipopolysaccharide (LPS)-activated RAW264.7 macrophages with the inhibition rate of 74.60% (40 μmol/L), while positive control dexamethasone gave 78.70% inhibition at 100 μg/ml.

Keywords:

• Ranunculaceae
• Aconitum bulleyanum
• bis-diterpenoid alkaloids
• anti-inflammation activity

Acknowledgements

We were grateful to the staff of analytical group at the State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences for the spectral data.