Biosynthesis and evaluation of a novel highly water-soluble quercetin glycoside derivative

Abstract

Quercetin (1) was converted into quercetin 7-O-succinyl glucoside (2) by used Bacillus amyloliquefaciens FJ18 as a solvent-resistant whole-cell biocatalyst. The structure of the new compound was confirmed by LC-MS analysis and NMR spectroscopy. The water-solubility of this novel quercetin 7-O-succinyl glucoside (2) was approximately 1000 times higher than that of native quercetin (2). Quercetin (1) and quercetin 7-O-succinyl glucoside (2) exhibited significant DPPH scavenging capacity with IC₅₀ values of 23.55 and 36.05 µM, respectively. Both compounds showed moderate cytotoxic effects against the two human cancer cell lines (MCF-7 and HepG2) with IC₅₀ values ranging from 39.45-63.38 µM.

Graphical Abstract

Keywords:

• Quercetin 7-O-glucoside derivative
• biotransformation
• Bacillus amyloliquefaciens
• water solubility
• DPPH
• cytotoxicity

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

This work was financially supported by the National Key Research and Development Program of China (No. 2019YFA0904900) and Tianjin Synthetic Biotechnology Innovation Capacity Improvement Project (No. TSBICIP-PTJS-003-04).