New depside and rosmarinic acid derivatives from Perilla frutescens and their anti-inflammatory activity

Abstract

Two new depside derivatives 1 and 2 as well as a new pair of rosmarinic acid enantiomers 3a/b were isolated from the leaves of Perilla frutescens (L.) britt. The chemical structures of these compounds were identified based on detailed spectroscopic and physicochemical analyses (HR-ESI-MS, NMR) and comparison of literature data. Compounds 3a/b were obtained by chiral separation, and their absolute configurations were determined by comparison of experimental and calculated ECD spectra. Compounds 3a/b exhibited potential inhibitory activity on nitric oxide (NO) production induced by lipopolysaccharide in RAW264.7 cells with IC₅₀ values of 15.92 ± 3.32 μM and 48.72 ± 4.12 μM.

Keywords:

• Perilla frutescens
• depside derivatives
• rosmarinic acid derivatives
• anti-inflammatory
• NO

Acknowledgements

We sincerely thank to support of the Funding and School of Chinese Materia Medica and State Key Laboratory of Component-Based Chinese Medicine, Tianjin University of Traditional Chinese Medicine.

Disclosure statement

No potential conflict of interest was reported by the author(s).

Additional information

Funding

This research was supported by the National Science and Technology Major Project of China (2017ZX09301012).