Preclinical determination of wound-healing activity of halibut oil cream in rat model of burn wound

Abstract

This study investigated the effects of halibut oil cream, containing omega-3 fatty acids, vitamins A and D, and hydroxyproline, on burn wound healing in rats. Acute dermal toxicity tests confirmed its nontoxicity. Wistar rats were divided into five groups: a control, a positive control treated with silver sulfadiazine 1% (SSD), and three groups treated with 3%, 9%, and 27% halibut oil cream Formulation (HBOF). The SSD and HBOF groups showed significant healing improvements compared to the control. Histopathological analysis indicated increased collagen production in the HBOF groups, suggesting halibut oil cream’s potential as a topical treatment for burn wounds.

Keywords:

• Halibut oil
• wound burn model
• wound area contraction
• acute dermal toxicity
• inflammatory phase
• proliferative phase

Acknowledgements

They are also thankful to Shri JJT University, Jhunjhunu, Rajasthan, for providing the necessary facilities and support to carry out this research project. The authors are also grateful to Dr. Avijit Choudhury, and Mr. Vishal Patel for their valuable support in carrying out the research work at Pretox Research Centre, Surat Gujarat.

Author contributions

STS identified the test formulation, generated resources, and did the in-vivo study execution and data analysis. AK supervised the project and gave final approval for the manuscript, SAA and DK performed the in-vitro experiments and manuscript reviewing. STS and DK prepared the formulation. SKD revised the overall manuscript. STS, AK, SAA, and SKD contributed to the final manuscript revision. AK and SKD supervised overall research project planning, generated resources, reviewed, and finally approved the manuscript.

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

The authors received no extramural funding for the study.