Abstract
Bioassay guided fractionation of Boenninghausenia sessilicarpa (Rutaceae) resulted in the isolation of a new dimeric coumarin glucoside 9′-methoxyl rutarensin (1) and a cytotoxic compound rutamarin (4), as well as an antivirus component leptodactylone (8), together with six known coumarins. Their structures were elucidated by 1D- and 2D NMR spectroscopy and ESI-MS analyses, respectively. Rutamarin (4) showed significant inhibitory activities against A-549, Bel-7402, HepG-2 and HCT-8 tumour cell lines with IC50s of 1.318, 2.082, 2.306 and 2.497 μg/ml. In addition, leptodactylone (8) showed potent protective activity on cells infected by SARS-CoV with ratio of 60% at 100 μg/ml.
Acknowledgements
The authors thank the Department of Instrumental Analysis of our institute for the measurement of UV, IR, NMR, and MS spectra. We also acknowledge Beijing Municipal Science and Technology Commission of China for financial support. In addition, we thank Mr Hongyan Liu for the cytotoxicity assays and the Shanghai Institute of Materia Medica for the antiviral assays.