The Recent Progress in DNAzymes-Based Aptasensors for Thrombin Detection

Abstract

Thrombin (TB) is classified among human blood coagulation proteins with key functions in hemostasis of blood vessels, wound healing, atherosclerosis, tissue adhesion, etc. Moreover, TB is involved as the main enzyme in the conversion of the fibrinogen to fibrin. Given the importance of TB detection in the clinical area, the development of innovative methods can considerably improve TB detection. Newly, aptasensors or aptamer-based biosensors have received special attention for sensitive and facile TB detection. In addition, the aptamer/nanomaterial conjugates have presented new prospects in accurate TB detection as nanoaptasensors. DNA-based enzymes or DNAzymes, as new biocatalysts, have many advantages over protein enzymes and can be used in analytical tools. This article reviews a brief overview of significant progresses regarding the various types of DNAzymes-based aptasensors and nano aptasensors developed for thrombin detection. In the following, challenges and prospects of TB detection by DNAzymes-based aptasensors are discussed.

Keywords:

• Aptamer
• aptasensor
• DNAzymes
• nanosensor
• thrombin

Disclosure statement

There are no conflicts to declare.

Notes

1 (2,2′-azino-bis(3 ethylbenzothiazoline)-6-sulfonic acid)

Additional information

Funding

The authors acknowledge the financial support from Mashhad University of Medical Sciences (MUMS), Mashhad, Iran.