Chemical constituent and bioactivity of Valeriana officinalis L. root essential oil using neutral cellulase-assisted steam distillation

ABSTRACT

Neutral cellulase-assisted steam distillation (NCSD) was developed to extract essential oil from Valeriana officinalis L. root, and a single-factor experiment was designed to optimize the NCSD. Chemical composition was subsequently compared between Valeriana officinalis L. root essential oil (VORO) extracted by NCSD and conventional steam distillation (SD). Results show that the highest yield of VORO extracted by NCSD was 68.09% higher than that extracted by SD. Results of antibacterial activity indicate that the two VOROs had an equally strong antibacterial activity of Bacillus subtilis, Staphylococcus aureus, and Escherichia coli with minimum inhibitory concentration (MIC) of 0.88, 1.75, and 3.50 mg/mL, respectively. Both VOROs also showed sufficiently high antioxidant and anti-dust mite abilities. The VORO concentration higher than 0.35 mg/mL was regarded as an A-grade dust mite repellent and B-grade miticide. This study has emphasized the significances of VORO in the food and textile industries as a potential additive.

KEYWORDS:

• Valeriana officinalis L. root essential oil
• neutral cellulase-assisted steam distillation
• antimicrobial activity
• antioxidant activity
• anti-dust mites activity

Acknowledgments

The following grants are gratefully acknowledged: Science and Technology Project of Market Supervision Administration of Jiangsu Province (KJ204132), National Nature Science Foundation of China (32001627 and 32172326), Key R&D Program of Jiangsu Province (BE2019362), National Training Program of Innovation and Entrepreneurship for Undergraduates (202110295035Z), and National first-class discipline program of Food Science and Technology (JUFSTR20180509). The corresponding author would like to thank the financial support from High-level Innovation and Entrepreneurship Talents Introduction Program of Jiangsu Province (Su Talent Office [2019] No. 20).

Disclosure statement

No potential conflict of interest was reported by the author(s).

Additional information

Funding

The work was supported by the Science and Technology Project of Market Supervision Administration of Jiangsu Province [KJ204132]; Key R&D Program of Jiangsu Province [BE2019362]; National Nature Science Foundation of China [32001627 and 32172326]; National Key R&D Program of China [2018YFC1602300].