A N-phosphoryl branched peptide (DIPP-Leu) 2 -Lys-OCH 3 was found to efficiently cleave double-strand DNA such as PUC19 and Lambda DNA at pH 8.5 in 100 mM Tris-HCl buffer. The T4 ligase experiment implied that the DNA cleavage occurs via a hydrolytic path.
Acknowledgments
The authors are very grateful for the National Natural Science Foundation of China (No: 20132020) for financial support of this project.