microRNAs as the biomarkers of chemotherapy-induced peripheral neuropathy in patients with multiple myeloma

Figures & data

Table 1. Clinical characteristics of the study groups.

	Group with neuropathy [N = 32]	Group without neuropathy [control group] [N = 28]	p-value*
	Mean ± SD	Mean ± SD
White blood cells (×10^3/µl)	6.69 ± 2.33	6.43 ± 3.85	0.17
Hemoglobin (g/dl)	11.51 ± 1.19	11.71 ± 1.50	0.87
Platelets (×10^3/µl)	198.20 ± 92.15	225.96 ± 115.24	0.30
Total protein (g/dl)	7.11 ± 1.62	6.09 ± 1.23	0.10
IgG (g/l)	17.01 ± 17.48	8.98 ± 5.22	0.48
IgA (g/l)	4.32 ± 11.53	6.60 ± 13.24	0.79
IgM (g/l)	0.38 ± 0.23	0.28 ± 0.14	0.45
FLCs kappa (mg/dl)	530.77 ± 2070.83	116.60 ± 156.32	0.98
FLCs lambda (mg.dl)	126.36 ± 553.64	26.86 ± 46.54	0.72
Sex (male/female)	19/13	20/8	0.42
Patient’sage (years)	66.65 ± 6.50	63.89 ± 10.03	0.32

*Mann–Whitney test except sex (Fisher’s exact test).

Table 2. Plasma miRNA profiles in peripheral neuropathy patients and controls.

	Group with neuropathy [N = 32]	Group without neuropathy [control group] [N = 28]	p-value*
	Mean ± SD	Mean ± SD
miRNA-191-5p	1334.90 ± 683.31	971.24 ± 658.87	0.024
miRNA-23a-3p	5264.05 ± 1678.83	3702.66 ± 2314.79	0.002
miRNA-26a-5p	2721.63 ± 1886.90	2428.72 ± 2394.15	0.197
miRNA-17-5p	1954.24 ± 535.71	1863.96 ± 693.56	0.358
miRNA-185-5p	1154.82 ± 494.92	1048.50 ± 265.90	0.573
miRNA-24-3p	3218.22 ± 1273.05	2022.66 ± 1221.85	0.0008
miRNA-92a-3p	9893.77 ± 5389.62	7526.91 ± 5527.82	0.0272
miRNA-22-3p	7885.72 ± 5060.10	4209.91 ± 2097.02	<0.0001
miRNA-486-5p	10,453.02 ± 10,866.44	10,158.32 ± 9113.01	0.614

*Mann–Whitney test; Expression was calculated in relation to the expression of miR-93 as a reference miRNA. In bold p-value < 0.05, which was considered statistically significant.

Table 3. ROC analysis results.

	AUC	Youden's index	p-value
miRNA191-5p	0.67	0.36	0.0185
miRNA23a-3p	0.731	0.44	0.0007
miRNA24-3p	0.751	0.44	0.0001
miRNA92	0.666	0.33	0.0191
miRNA22	0.807	0.49	0.00000003

In bold p-value <0.05, which was considered statistically significant.

AUC: area under curve.

Table 4. Multivariate logistic regression models with logarithmically transformed miRNA expression levels as independent variables and neuropathy as dependent variable.

	Odds ratio	−95% CL	+95% CL	p-value
Model 1
Log miRNA-24a-3p	6.17	1.29	29.47	0.019
Log miRNA-22-3p	10.38	2.23	48.34	0.002
Model 2
Log miRNA-191-5p	5.78	1.32	25.15	0.016
Log miRNA-22-3p	33.89	4.08	281.35	0.0008
Model 3
Log VTD	5.22	0.93	29.18	0.05
Log miRNA-191-5p	5.09	1.05	25.25	0.037
Log miRNA-22-3p	38.67	3.64	409.81	0.001
Model 4
Log VTD	7.77	1.28	47.06	0.022
Log miRNA-24a-3p	7.4	1.21	44.93	0.026
Log miRNA-22-3p	12.13	2.2	66.79	0.003