Middle-thermophilic sulfur-oxidizing bacteria Thiomonas sp. RAN5 strain for hydrogen sulfide removal

Figures & data

Figure 1. Schematic of H₂S removal from artificial gas.

Figure 2. Schematic of H₂S removal from anaerobically digested gas.

Table 1. Characteristics of isolates and related species

	RAN5	T. thermosulfata*	T. perometabolis*	T. intermedia*
Morphology	Rod	Rod	Rod	Rod
Gram stain	−	−	−	−
Motility	+	+	+	+
Isolation and optimum pH	7.0	4.3–7.8	5.0–7.0	5.0–7.5
Isolation and optimum temperature (° C)	45	34–65	15–42	15–37
Growth on:
Thiosulfate	+	+	+	+
Sulfide	+	+	+	+
Elemental sulfur	+	+	+	+
Tetrathionate	+	+	+	+
Anaerobic growth	−	−	−	−
Optimum growth on mixotrophic medium	+	+	+	+
Heterotrophic growth	−	−	−	−
Notes: + = positive; − = negative. *Kelly and Wood (2005).

Figure 3. Influence of medium pH and incubation temperature on sulfur oxidization by the RAN5 strain.

Figure 4. Influence of contamination with environmental microorganisms on H₂S removal. Open circle: compost + RAN5; open square: RAN5; open triangle: compost.

Figure 5. Efficiency of H₂S removal from anaerobically digested gas by strain RAN5. Arrow: influent H₂S concentration greater than 2000 ppm.

Table 2. Numbers of SOB in livestock wastewater inoculated with the RAN5 strain (MPN/mL)

Sample	Day 0	Day 1	Day 3
Livestock wastewater	n.d.	n.d.	n.d.
Livestock wastewater + RAN5	1.5 × 10^3	1.5 × 10^2	4.3 × 10^1
Sterilized livestock wastewater + RAN5	9.3 × 10^3	4.3 × 10^2	4.3 × 10^1
Note: n.d. = not detected.

Table 3. Amount of sulfate in livestock wastewater inoculated with the RAN5 strain. (SO₄ ²⁻ mM)

Sample	Day 0	Day 1	Day 3
Livestock wastewater	0.09	0.06	0.04
Livestock wastewater + RAN5	0.25	0.20	0.06
Sterilized livestock wastewater + RAN5	0.24	0.23	0.27

Kelly , D.P. and Wood , A.P. 2005 . “ Genus Thiomonas ” . In Bergey's Manual of Systematic Bacteriology , 2nd , Edited by: Garrity , G.M. and Part , C. Vol. 2 , 757 – 758 . New York : Springer .

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