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Technical Papers

Effect of biocover equipped with a novel passive air diffusion system on microbial methane oxidation and community of methanotrophs

, , , , &
Pages 278-286 | Published online: 24 Feb 2012

Figures & data

Figure 1. Simulated landfill biocover microcosms (left, MSCBS; right, MSNBS).

Figure 1. Simulated landfill biocover microcosms (left, MSCBS; right, MSNBS).

Table 1. Methanotroph-specific primers and probes

Figure 2. O2 content in different microcosm layers at different SLFG flow rates. (a) MSCBS; (b) MSNBS.

Figure 2. O2 content in different microcosm layers at different SLFG flow rates. (a) MSCBS; (b) MSNBS.

Figure 3. CH4 content in different microcosm layers at different SLFG flow rates. (a) MSCBS; (b) MSNBS.

Figure 3. CH4 content in different microcosm layers at different SLFG flow rates. (a) MSCBS; (b) MSNBS.

Figure 4. Fingerprints of type I (a) and type II (b) methanotrophic bacterial communities in the microcosms as generated by nested PCR-DGGE (denaturing gradient: 30–60%). Bands designated with an asterisk were excised and subjected to sequencing and identification.

Figure 4. Fingerprints of type I (a) and type II (b) methanotrophic bacterial communities in the microcosms as generated by nested PCR-DGGE (denaturing gradient: 30–60%). Bands designated with an asterisk were excised and subjected to sequencing and identification.

Figure 5. Neighbor-joining tree depicting phylogenetic relationships of type I methanotrophic populations detected in microcosms by nested PCR-DGGE.

Figure 5. Neighbor-joining tree depicting phylogenetic relationships of type I methanotrophic populations detected in microcosms by nested PCR-DGGE.

Table 2. Comparison of the microbial diversity and dissimilarity of type I and type II methanotrophics between reactors A and B

Figure 6. Neighbor-joining tree depicting phylogenetic relationships of type II methanotrophic populations detected in microcosms by nested PCR-DGGE.

Figure 6. Neighbor-joining tree depicting phylogenetic relationships of type II methanotrophic populations detected in microcosms by nested PCR-DGGE.
Supplemental material

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