Figures & data
Figure 1. Histomorphology of longissimus dorsi muscle and circRNAs distribution analysis. (A), HE staining of longissimus dorsi tissue of DBG embryo at day 75; (B), HE staining of longissimus dorsi tissue of DBG 1 day after birth; (C), distribution of all identified circRNAs on chromosomes; (D), Principal component analysis (PCA) was performed on all identified circRNAs in the longissimus dorsi muscle of 12 DBGs.
![Figure 1. Histomorphology of longissimus dorsi muscle and circRNAs distribution analysis. (A), HE staining of longissimus dorsi tissue of DBG embryo at day 75; (B), HE staining of longissimus dorsi tissue of DBG 1 day after birth; (C), distribution of all identified circRNAs on chromosomes; (D), Principal component analysis (PCA) was performed on all identified circRNAs in the longissimus dorsi muscle of 12 DBGs.](/cms/asset/eef5a441-6195-4423-908b-2137475fc5f1/labt_a_2286609_f0001_c.jpg)
Table 1. Comparison of abbreviations and full names.
Figure 2. Analysis of circRNA expression in two stages of skeletal muscle development of DBG: (a), clustering heatmap of DEcircRNA and DE_HGs; (B), schematic diagram of obtaining the full length of circRNA by PCR amplification; (C), agarose gel electrophoresis of PCR amplification products; (D), expression levels of circRNA (RPM) by RNA_seq; (E), relative expression level of circRNA by RT_qPCR; “*”: P < 0.05; “**”: P < 0.01.
![Figure 2. Analysis of circRNA expression in two stages of skeletal muscle development of DBG: (a), clustering heatmap of DEcircRNA and DE_HGs; (B), schematic diagram of obtaining the full length of circRNA by PCR amplification; (C), agarose gel electrophoresis of PCR amplification products; (D), expression levels of circRNA (RPM) by RNA_seq; (E), relative expression level of circRNA by RT_qPCR; “*”: P < 0.05; “**”: P < 0.01.](/cms/asset/75f38f64-e7aa-42c2-b5aa-e423f05110b4/labt_a_2286609_f0002_c.jpg)
Supplemental Material
Download Zip (1.3 MB)Data availability statement
All data of RNA_seq data was cited and download from NCBI database (PRJNA749391).