Morpometric and molecular characterization of Surguli goat through CO1 gene in district Kohat

Figures & data

Figure 1. (a) Surguli buck, (b) Surguli doe.

Table 1. Effect of sex on the body weight (kg) and morphometric measurements (cm) of Surguli goats.

Parameters	Mean ± SE		p-Value
	Male	Female
Body length	56.38^a±0.70	53.45^b±0.53	0.0026
Body weight	34.25^a±0.49	32.21^b±0.37	0.0028
Heart girth	81.06^a±0.16	80.83^b±0.12	0.0059
Height at wither	74.31 ± 1.25	70.63 ± 1.64	0.0858
Height at rump	77.09 ± 1.52	74.21 ± 1.15	0.1429
Head length	12.09 ± 0.83	10.73 ± 0.63	0.2093
Ear length	9.36 ± 1.23	8.47 ± 0.94	0.5723
Horn length	11.89^a±0.48	8.00^b±0.25	0.0092
Neck length	21.54 ± 0.63	22.05 ± 0.48	0.5324
Tail length	13.40 ± 0.46	13.37 ± 0.35	0.9713

Mean values in a row with different superscripts are different at (p < 0.05).

Table 2. Effect of age on the body weight (kg) and morphometric measurements (cm) of Surguli goats (mean ± SE).

Parameters	2 Year	3 Year	4 Year	5 Year	p-Value
Body length	54.25 ± 0.98	54.37 ± 1.38	54.51 ± 0.87	55.40 ± 0.98	0.6726
Body weight	32.92 ± 0.62	32.92 ± 0.62	33.45 ± 0.69	32.15 ± 0.98	0.7586
Heart girth	80.52 ± 0.19	80.77 ± 0.20	80.80 ± 0.22	80.85 ± 0.31	0.6307
Height at wither	70.62 ± 1.86	72.60 ± 1.66	72.62 ± 1.70	74.70 ± 2.63	0.0864
Height at rump	70.75^b±1.50	74.90^a±1.34	78.25^a±1.80	79.25^a±2.12	0.0046
Head length	11.00 ± 1.04	11.60 ± 0.93	11.65 ± 1.47	11.80 ± 1.53	0.9679
Ear length	7.37^b±1.29	7.60^b±1.15	9.25^ab±1.29	13.75^a±1.83	0.0383
Horn length	6.66^b±0.31	7.07^b±0.27	8.48^a±0.31	8.87^a±0.44	0.0002
Neck length	21.00 ± 0.74	21.80 ± 0.66	22.25 ± 0.84	23.00 ± 1.05	0.4397
Tail length	12.80^c±0.47	13.00^b±0.42	13.50^ab±0.47	15.30^a±0.66	0.0284

Mean values in a row with different superscripts are different at (p < 0.05).

Table 3. Equations for predicting live weight of Surguli goat from body parameters.

Prediction equation	Adj. R^2 (%)	p-Value	SE		Ind. variable
			b0	b1
−3.95 + 0.67x	92	0.0001	1.11	0.02	Body length
−132.4 + 2.05x	44	0.0001	19.35	0.23	Hearth girth

Adj. R² (%) = adjusted R² (%); SE: standard error; Ind. variable: independent variable.

Table 4. SNPs positions in nucleotides sequence of CO1 gene with reference sequence Capra hircus mitochondrial genome accession no (KP662714.1).

Polymorphic sites
		5	8	10	12	13	33	260	350	756
1	KP662714	G	T	G	T	A	C	C	T	G
2	M33	.	.	T	.	.	.	.	C	.
3	M34	.	.	T	.	G	T	.	.	.
4	M35	.	.	.	.	.	T	.	.	.
5	M36	.	.	.	.	.	T	.	.	.
6	M37	.	C	.	.	.	T	T	.	.
7	M38	A	.	.	.	.	T	.	.	A
8	M39	.	C	T	.	.	T	.	C	.
9	M40	.	.	.	.	.	T	.	C	.
10	M41	.	.	.	G	.	T	.	.	.
11	M42	.	C	T	.	.	.	.	.	.

M33…M42: tag no of surguli goat samples in which SNPs were identified; A: adenine; C: cytosine; G: guanine; T: thymine.

Table 5. Intraspecific diversity within CO1 sequences of the samples (M33-M42) and interspecific diversity with CO1 sequences of closely related species with specific accession numbers assembled from NCBI.

	1	2	3	4	5	6	7	8	9	10	11	12	13	14
KP662714
M33	0.0025
M34	0.0038	0.0038
M35	0.0012	0.0038	0.0025
M36	0.0012	0.0038	0.0025	0.0000
M37	0.0038	0.0063	0.0050	0.0025	0.0025
M38	0.0038	0.0063	0.0050	0.0025	0.0025	0.0050
M39	0.0050	0.0025	0.0038	0.0038	0.0038	0.0038	0.0063
M40	0.0025	0.0025	0.0038	0.0012	0.0012	0.0038	0.0038	0.0025
M41	0.0025	0.0050	0.0038	0.0012	0.0012	0.0038	0.0038	0.0050	0.0025
M42	0.0025	0.0025	0.0038	0.0038	0.0038	0.0038	0.0063	0.0025	0.0050	0.0050
MG603753.Qaidem	0.0014	0.0028	0.0014	0.0014	0.0014	0.0028	0.0028	0.0028	0.0028	0.0014	0.0014
KJ940969.tibetan	0.0014	0.0000	0.0014	0.0014	0.0014	0.0028	0.0028	0.0000	0.0000	0.0014	0.0014	0.0019
KM244714.Meigu	0.0014	0.0000	0.0014	0.0014	0.0014	0.0028	0.0028	0.0000	0.0000	0.0014	0.0014	0.0019	0.0000
NC_020623. Capra_Ibex	0.0295	0.0281	0.0295	0.0295	0.0295	0.0309	0.0309	0.0281	0.0281	0.0295	0.0295	0.0310	0.0304	0.0304

Figure 2. Phylogenetic tree constructed by N–J method and Tamura-Nei model in MEGAX software from CO1 haplotypes found in the present study and previously published haplotypes of other related species assembled from NCBI.