Temperature adaptation patterns in Chinese cattle revealed by TRPM2 gene mutation analysis

Figures & data

Figure 1. Sequencing map for NC_037328.1: c.805A > G:p. Met269Val locus of the TRPM2 gene.

Table 1. Results of correlation analysis of annual mean temperature, humidity, and humidity-temperature index.

Locus	Genotype(number)	Temperature (°C) (LSM ± SE)	Humidity (％) (LSM ± SE)	THI (LSM ± SE)
NC_037328.1: c.805A > G:p.Met269 Val	AA(151)	9.4079^A ± 0.376	64.401^A ±0.588	50.903^A ±0.545
	AG(144)	14.408^C ±0.449	72.292^B ±0.751	58.308^B ±0.685
	GG(112)	13.191^B ±0.281	77.853^C ±0.468	61.721^C ±0.632

Note: Superscripts ^{A, B,} and ^C in the above table represent significant differences among genes (P < 0.01).

Figure 2. Distribution of a and G alleles and AA, AG, and GG genotypes in 20 Chinese cattle breeds.

Table 2. Species, population, and genotyping tables for a sample of 407 individuals.

Geogcalraphi	Breed	Genotype frequencies			Allele frequencies
Grouping		AA	AG	GG	A	G
Northern group	Yanbian (YB)	1.0000(20)	0.0000(0)	0.0000(0)	1.0000	0.0000
	Tibetan (TB)	0.8667(13)	0.1333(2)	0.0000(0)	0.9333	0.0667
	Yushu (YS)	0.7857(11)	0.2143(3)	0.0000(0)	0.8929	0.1071
	Kazak (HSK)	0.7500(15)	0.2500(5)	0.0000(0)	0.8750	0.1250
	Mongolian (MG)	0.6000(12)	0.4000(8)	0.0000(0)	0.8000	0.2000
Central group	Qinchuan (QC)	0.8000(8)	0.2000(2)	0.0000(0)	0.9000	0.1000
	Zaosheng (ZS)	0.6500(13)	0.3000(6)	0.0500(1)	0.8000	0.2000
	Bohai (BH)	0.5000(15)	0.4667(14)	0.0333(1)	0.7333	0.2667
	Jiaxian (JX)	0.4500(9)	0.5000(10)	0.0500(1)	0.7000	0.3000
	Luxi (LX)	0.4667(14)	0.3000(9)	0.2333(7)	0.6167	0.3833
Southern group	Wannan (WN)	0.1000(2)	0.3500(7)	0.5500(11)	0.2750	0.7250
	Wenshan (WS)	0.0000(0)	0.2000(6)	0.1000(24)	0.1000	0.9000
	Dabie (DB)	0.1176(2)	0.4118(7)	0.4706(8)	0.3235	0.6765
	Hainan (HN)	0.0741(2)	0.5185(11)	0.4074(14)	0.3333	0.6667
	Weizhou(WZ)	0.1000(2)	0.4500(9)	0.4500(9)	0.3250	0.6750
	Lingnan (LN)	0.2000(6)	0.4667(14)	0.3333(10)	0.4333	0.5667
	Weining (WN)	0.2778(5)	0.3333(6)	0.3889(7)	0.4444	0.5556
	Sanjiang (SJ)	0.0500(1)	0.5000(10)	0.4500(9)	0.3000	0.7000
	Longling (LL)	0.0500(1)	0.5000(10)	0.4500(9)	0.3000	0.7000
	Dehong (DH)	0.0000(0)	0.1667(1)	0.8333(5)	0.0833	0.9167
	proportion				0.5479	0.4521

Figure 3. Protein structural changes.

Figure 4. QMEAN Z Score results.