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Research Articles

Toll like receptor 4 (TLR4) gene polymorphism and its association with somatic cell score and milk production traits in Indian dromedary camels

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Figures & data

Table 1. Details of primers used for PCR amplification of TLR4 gene in Indian camel.

Figure 1. PCR amplification of TLR4 gene fragment spanning 5’ UTR, exon 1 and partial intron 1 region (573 bp), resolved on 1.5% agarose gel (M – Molecular weight marker = 100 bp).

Figure 1. PCR amplification of TLR4 gene fragment spanning 5’ UTR, exon 1 and partial intron 1 region (573 bp), resolved on 1.5% agarose gel (M – Molecular weight marker = 100 bp).

Figure 2. Sequence chromatogram of different genotypes at C472T SNP locus identified in intron-1 region of TLR4 gene.

Figure 2. Sequence chromatogram of different genotypes at C472T SNP locus identified in intron-1 region of TLR4 gene.

Table 2. Genotype and allele frequency of the studied TLR4 gene fragment in Indian Dromedary camel.

Figure 3. Phylogenetic tree constructed using Neighbor-Joining method.

Figure 3. Phylogenetic tree constructed using Neighbor-Joining method.

Table 3. Comparison of nucleotide sequence of Indian Dromedary Camel TLR4 gene (Accession No. OM621865) with other species.

Figure 4. Microscopic examination of milk somatic cells smear stained with Newman stain showing somatic cells (numerous milk somatic cells present in milk smear).

Figure 4. Microscopic examination of milk somatic cells smear stained with Newman stain showing somatic cells (numerous milk somatic cells present in milk smear).

Table 4. Somatic cell count and somatic cell score data recorded in the population with mean values.

Table 5. Least squares means of somatic cell score, lactation yield and peak yield in Indian Dromedary Camel.