Figures & data
Table 1. PCR primer sequences of the sheep E2F8 gene for amplification.
Figure 1. The research design: the identification of the sheep E2F8 gene was achieved through a comprehensive genome-wide association study (GWAS). Subsequently, we conducted an exhaustive search across the ensemble and NCBI databases to explore potential genetic loci and designed five primer pares. This comprehensive approach led to the discovery of the P1-del-32bp InDel variant. Furthermore, this was followed by PCR analysis to confirm our findings. Agarose gel electrophoresis (3.5%) of allele specific polymerase chain reaction (PCR) product and sequencing map of the P1-del-32bp InDel variant within the E2F8 gene in AUW sheep. II: homozygous insertion genotype; DD: homozygous deletion genotype; ID: heterozygous insertion/deletion genotype. The sequence with the black border is the difference sequence. The top band in the electrophoresis pattern of ID represented heteroduplex.
Table 2. Genetic diversity parameters for E2F8-P1-del-32bp locus in AUW sheep population.
Table 3. Associations of E2F8-P1-del-32bp with litter size in different parity groups in AUW ewes.