Age-dependent changes in the expression and localization of LYZL4, LYZL6 and PCNA during testicular development in the Ashidan yak

Figures & data

Table 1. Details of primer sequences used for qRT-PCR.

Primer name	Primer sequence	Product size (bp)
LYZL4	F: ACCCTATGGCTGTCTATGAA R: TGGATTCAGTAAAGCAGAGC	131
LYZL6	F: GTGCCTGGCTTTCGTAGAGA R: CGTGCGACTCTGGTAATCGT	127
PCNA	F: GTGTCATTGCGACTCCG R: AACACTGCCTACAACG	121
GAPDH	F: GGGGAAAAGCGGACTTAGGA R: TGGTTCACGCCCATCACA	143

Figure 1. Relative expression of LYZL4, LYZL6 and PCNA mRNA in testis of the yak; different letters indicate significant difference (p < 0.01).

Figure 2. Expression of LYZL4, LYZL6 and PCNA protein in testis of the yak; (A) the LYZL4, LYZL6 and PCNA detected by Western blotting; (B) The protein expression of LYZL4, LYZL6 and PCNA; different letters indicate significant difference (p < 0.05).

Figure 3. Immunohistochemical staining of LYZL4 protein at different developmental stages of yak testes (400×); (A) 6 months of Ashidan yak; (B) 18 months of Ashidan yak; (C) 30 months of Ashidan yak; (D) 72 months of Ashidan yak.

Figure 4. Immunohistochemical staining of LYZL6 protein at different developmental stages of yak testes (400×); (A) 6 months of Ashidan yak; (B) 18 months of Ashidan yak; (C) 30 months of Ashidan yak; (D) 72 months of Ashidan yak.

Figure 5. Immunohistochemical staining of PCNA protein at different developmental stages of yak testes (400×); (A) 6 months of Ashidan yak; (B) 18 months of Ashidan yak; (C) 30 months of Ashidan yak; (D) 72 months of Ashidan yak.