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ABSTRACT
A novel method to prepare capillary coatings using dopamine (DA) and hyaluronic acid (HA) was mentioned in this paper. Firstly, dopamine was oxidized to form polydopamine and attached to the inner wall of the capillary spontaneously. HA attached to the polydopamine by electrostatic interaction to form a polydopamine-hyaluronic acid (PDA-HA) coating. Protein adsorption on the inner surface of capillary was suppressed, and the proteins of lysozyme (Lys), cytochrome c (Cyt-c), bovine serum albumin (BSA) and ribonuclease A (RNase A) were baseline separated obviously using capillary electrophoresis (CE) within 20 minutes.
Funding
This work is financially supported by the National Natural Science Foundation of China (21375069, 21574072, 21675091, 21404065), the Natural Science Foundation for Distinguished Young Scientists of Shandong Province (JQ201403), the Taishan Young Scholar Program of Shandong Province (tsqn20161027), the Key Research and Development Project of Shandong Province (2016GGX102028, 2016GGX102039), the Project of Shandong Province Higher Educational Science and Technology Program (J15LC20), the Scientific Research Foundation for the Returned Overseas Chinese Scholars of State Education Ministry (20111568), the People's Livelihood Science and Technology Project of Qingdao (166257nsh), and the Postdoctoral Scientific Research Foundation of Qingdao.