Preparation and Characterization of Goat Hoof Keratin/Gelatin/Sodium Alginate Base Biofilm for Tissue Engineering Application

Abstract

The formation of keratin (KE) based tissue regenerative substitute from waste goat hoof source, integrated with gelatin (GE) and sodium alginate (AL) for improved biological and mechanical properties. In this study, biofilm samples prepared using solvent casting method showed improved physiochemical and morphological properties. The biofilm was characterized by FTIR spectra, XRD spectra, TG-DTA curve and SEM analysis. During in-vitro swelling and antimicrobial study, the biofilm exhibits good swelling and antibacterial property. The biofilm also reveals excellent biocompatibility in fibroblast cell lines. KE based biofilm has good cell binding nature and used as rapid tissue growth promoter in tissue engineering applications.

Keywords:

• Biofilm
• natural biomaterial
• Hoof keratin
• tissue regeneration
• wound healing

Acknowledgements

Author thanks DBT, GOI for Providing International Travel Grant to attend International Conference in USA, Ref Number: DBT/CTEP/02/201601450 (Dated on 25/11/2016). I also thank the institution for providing the facilities of Biomedical Research Lab.