Optimisation of Ferula assa-foetida -Loaded PLGA Nanoparticles Synthesised and evaluation of putative mechanism for anticancer properties

ABSTRACT

   

Loading of Ferula assa-foetida essential oil on PLGA nanoparticles for improve its biological activity.

FAFEO-PNP were produced by W₁/O/W₂ method and after characterization, its antibacterial (Disc Diffusion, MIC, MBC), antioxidant (ABTS, DPPH, FRAP), pro-apoptotic (MTT, qPCR, AO/PI staining, Flow cytometery) and anti-angiogenic (CAM) effects were investigated. Cancer induction was performed to evaluate the antitumor effects of FAFEO-PNP.

FAFEO-PNP (size: 203.62 nm, PDI: 0.26 and ζ-p: -32.92 mV) inhibited HepG2 (IC₅₀: 57μg/mL) cells. FAFEO-PNP induced the cell death by up-regulation of P53, TNF-α, P-21, Cas-3 and 9 genes. The activity of inhibiting free radicals and inhibiting the growth of different strains of bacteria in the presence of nanoparticles showed the antioxidant and antibacterial power of FAFEO-PNP. In vivo analysis including CAM and cancer induction assay confirmed the anti-angiogenic (reduction of vascular parametric factors) and anti-tumor effects of FAFEO-PNP. FAFEO-PNP can be considered as an anti-cancer agent in cancer therapy.

KEYWORDS:

• Ferula assa-foetida
• PLGA nanoparticles
• antioxidant
• encapsulation
• anti-angiogenic
• anti-cancer

Acknowledgments

This work was supported by, Islamic Azad University, Mashhad, Iran and thus is appreciated by the author.

Disclosure statement

No potential conflict of interest was reported by the author(s).