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Abstract
The oxidative modification of LDL may play an important role in the early events of atherogenesis. Thus the identification of antioxidative compounds may be of therapeutic and prophylactic importance regarding cardiovascular disease. Copper-chlorophyllin (Cu-CHL), a Cu2+-protoporphyrin IX complex, has been reported to inhibit lipid oxidation in biological membranes and liposomes. Hemin (Fe3+-protoporphyrin IX) has been shown to bind to LDL thereby inducing lipid peroxidation. As Cu-CHL has a similar structure as hemin, one may assume that Cu-CHL may compete with the hemin action on LDL. Therefore, in the present study Cu-CHL and the related compound magnesium-chlorophyllin (Mg-CHL) were examined in their ability to inhibit LDL oxidation initiated by hemin and other LDL oxidizing systems. LDL oxidation by hemin in presence of H2O2 was strongly inhibited by both CHLs. Both chlorophyllins were also capable of effectively inhibiting LDL oxidation initiated by transition metal ions (Cu2+), human umbilical vein endothelial cells (HUVEC) and tyrosyl radicals generated by myeloperoxidase (MPO) in presence of H2O2 and tyrosine. Cu- and Mg-CHL showed radical scavenging ability as demonstrated by the diphenylpicrylhydracylradical (DPPH)-radical assay and estimation of phenoxyl radical generated diphenyl (dityrosine) formation. As assessed by ultracentrifugation the chlorophyllins were found to bind to LDL (and HDL) in serum. The present study shows that copper chlorophyllin (Cu-CHL) and its magnesium analog could act as potent antagonists of atherogenic LDL modification induced by various oxidative stimuli. As inhibitory effects of the CHLs were found at concentrations as low as 1 μmol/l, which can be achieved in humans, the results may be physiologically/therapeutically relevant.
| Abbreviations | ||
| Cu-CHL | = | copper-chlorophyllin |
| Mg-CHL | = | magnesium-chlorophyllin |
| HUVEC | = | human umbilical vein endothelial cells |
| DPPH | = | diphenylpicrylhydracylradical |
| MPO | = | myeloperoxidase |
| TBARS | = | thiobarbituric reactive substances |
| TF | = | tissue factor |
| Abbreviations | ||
| Cu-CHL | = | copper-chlorophyllin |
| Mg-CHL | = | magnesium-chlorophyllin |
| HUVEC | = | human umbilical vein endothelial cells |
| DPPH | = | diphenylpicrylhydracylradical |
| MPO | = | myeloperoxidase |
| TBARS | = | thiobarbituric reactive substances |
| TF | = | tissue factor |