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Abstract
This paper presents Q-band electron spin resonance (ESR) studies on free radicals (FR) generated in potato leaves exposed to different O3 levels in open-top chambers (OTC), together with a quantitative study of the relationship between FR signal intensity and area of potato leaf damage. The advantages of Q-band when compared to X-band ESR spectroscopy are analysed, the main advantage being an absence of overlapping between Mn(II) and FR signals, allowing a quantitative study of FR signal intensity. This study also reports on a graphical method developed to quantitatively measure the damaged area on leaves caused by ozone exposure. Results indicate a direct relationship between FR signal intensity (measured as area under the signal) and percentage of O3 damage and clearly demonstrate a close relationship between visible ozone-induced symptoms and permanent FR concentration in potato leaves.
| Abbreviations | ||
| auc | = | area under the curve of the ESR signal |
| ESR | = | electron spin resonance |
| FR | = | free radical(s) |
| I | = | nuclear spin |
| id | = | intensity of damage |
| R-squared | = | determination coefficient |
| RMSE | = | root mean squared error |
| OTC | = | open-top chambers |
| UTC | = | universal time coordinated |
| Abbreviations | ||
| auc | = | area under the curve of the ESR signal |
| ESR | = | electron spin resonance |
| FR | = | free radical(s) |
| I | = | nuclear spin |
| id | = | intensity of damage |
| R-squared | = | determination coefficient |
| RMSE | = | root mean squared error |
| OTC | = | open-top chambers |
| UTC | = | universal time coordinated |