Surface-Modified Mesoporous Ceramics as Delivery Vehicle for Haemoglobin

Figures & data

Langmuir and Freundlich adsorption parameters of sugars on hydroxy apatite

Sugar	b	ym	log K	n	rF	rL
Cellobiose	6.89	3.38	2.446	1.794	0.966	0.990
Maltose	6.54	3.77	2.448	2.897	0.963	0.999
Sucrose	2.91	4.90	2.414	1.807	0.937	0.990
Trehalose	2.13	4.91	2.447	1.747	0.983	0.980

b, binding constant; y_m, amount adsorbed in mg/gm; r_F, correlation coefficient for Freundlich isotherm; r_L, correlation coefficient for Langmuir isotherm.

1 Concanavalin-induced aggregation studies: plots of absorbance against time of aggregation of sugar-coated ceramics dried by various methods.

Langmuir and Freundlich adsorption parameters of haemoglobin on sugar coated hydroxy apatites

Sugar	b	ym	log K	n	rF	rL
Cellobiose	2.845	134.8	0.972	2.004	0.986	0.874
Maltose	5.03	121.3	0.963	2.175	0.959	0.996
Sucrose	4.22	111.9	1.001	2.375	0.982	0.999
Trehalose	9.53	99.3	1.0	3.605	0.951	0.999

b, binding constant; y_m, amount adsorbed in mg/gm; r_F, correlation coefficient for Freundlich isotherm; r_L, correlation coefficient for Langmuir isotherm.

2 Photomicrograph of haemoglobin aquasome formulation (aggregates).

3 Photomicrograph of haemoglobin aquasome formulation (individual particle).

Physical characteristics of haemoglobin adsorbed formulations

Sugar	Size	Viscosity (cps)	Hb loading % (n)
Cellobiose	196 ± 13 μm	65.1	7.53
Maltose	205 ± 21 μm	64.6	7.29
Sucrose	198 ± 22 μm	65.5	7.36
Trehalose	201 ± 19 μm	64.0	7.42

* Shear rate, 1000 rpm.

4 Graph showing the oxygen dissociation curves for HAF, fresh blood and Hb solution.

5 The Hill's plots for HAF, fresh blood, and Hb solution.

Stability studies data of formulations

Time (days)	Total Hb %	Hb desorbed %	% of total methaemoglobin Hb
1	7.32	0.16	9.60
7	7.29	0.73	10.32
15	7.13	1.02	11.56
30	7.04	1.64	12.40

Hematocrit dilution curve for continuous isovolemic exchange transfusion in rats

	Hematocrit % on exchange transfusion with
Time of exchange (min)	HAF	Dextran (5.5% w/v)
0	46	45
10	41	38
20	40	33
30	35	30
40	34	26
50	32	23
60	31	20
70	30	18
80	28	12
90	27	10**
100	23	7.6**

* no mortality;

** mortality appeared;

*** 100% mortality.

The effect of 50% isovolemic exchange transfusion on arterial systolic blood pressure and heart rate

	Blood pressure (mm of Hg) on exchange with		Heart rate on exchange with
Hematocrit %	HAF	Dextran (5.5% w/v)	HAF	Dextran (5.5% w/v)
46	117.0 ± 5.0	115.6 ± 2.2	320 ± 8	318 ± 2
40	115.0 ± 4.2	114.3 ± 3.7	318 ± 10	317 ± 0
34	115.4 ± 4.0	113.7 ± 4.8	317 ± 9	310 ± 6
28	114.0 ± 5.0	111.9 ± 4.6	319 ± 7	313 ± 7
23	116.3 ± 4.2	111.7 ± 4.9	319 ± 7	304 ± 7