Solid Lipid Nanoparticles Containing Tamoxifen Characterization and In Vitro Antitumoral Activity

Figures & data

TABLE 1 Dimensional analysis by PCS and drug-loading capacity of tamoxifen-loaded SLN prepared by warm oil-in-water microemulsion (SLN-m) and by precipitation method (SLN-p)

	Size (nm)	PI	Tamoxifen incorporated (% w/w)
SLN-m	118 ± 3	0.16 ± 0.02	30.8
SLN-p	69 ± 2	0.12 ± 0.01	28.3

PI = polydispersity index. Each value is the mean of 5 experiments ±SE.

TABLE 2 Zeta potential values (mV) of the tamoxifen-loaded SLN prepared by warm oil-in-water microemulsion (SLN-m) and by precipitation method (SLN-p)

	H2O	NaCl	Phosphate buffer pH 7.4
SLN-m	−39.6 ± 1.6	−19.8 ± 0.7	−22.3 ± 1.3
SLN-p	−44.8 ± 1.0	−19.5 ± 0.7	−22.5 ± 2.5

Each value is the mean of 3 experiments ±SE.

FIG. 1 Tamoxifen stability at 37°C in human plasma. Each value is the mean of three experiments. All calculated SE were less than 3% of the mean values and then they were not reported.

FIG. 2 Release profiles of tamoxifen at pH 7.4 and in plasma from nanoparticles prepared by precipitation technique (SLN-p). Each value is the mean of three experiments. All calculated SE were less than 3% of the mean values and then they were not reported.

FIG. 3 Release profile of tamoxifen at pH 1.1 from nanoparticles prepared by precipitation technique (SLN-p). Each value is the mean of three experiments. All calculated SE were less than 3% of the mean values and then they were not reported.

FIG. 4 Amount of tamoxifen entrapped in the SLN prepared by precipitation technique (SLN-p) as function of release time at pH 1.1. Each value is the mean of three experiments. All calculated SE were less than 3% of the mean values and then they were not reported.

FIG. 5 Release profile of tamoxifen into octanol phase from nanoparticles prepared by precipitation technique (SLN-p). Each value is the mean of three experiments. All calculated SE were less than 3% of the mean values and then they were not reported.

FIG. 6 Antiproliferative activity of free tamoxifen and tamoxifen-loaded SLN, prepared by precipitation technique (SLN-p), on human breast cancer MCF-7 cells. Each value is the mean of three experiments. All calculated SE were less than 3% of the mean values and then they were not reported.