Evaluation of Pharmasolve^® corneal permeability enhancement and its irritation on rabbit eyes

Figures & data

Table 1.  Solubility of the four test drugs in PBS at pH 7.4 with different concentrations of Pharmasolve^®.

	Ribavirin (mg/mL)	Puerarin (mg/mL)	Enoxacin (mg/mL)	Ibuprofen (mg/mL)
0% Pharmasolve^®	314.21	8.13	0.61	2.74
2.5% Pharmasolve^®	404.76	13.92	0.64	5.84
5% Pharmasolve^®	477.44	16.19	0.81	6.12
10% Pharmasolve^®	1142.26	38.88	1.20	6.68

Figure 1.  Corneal permeability coefficients change of ribavirin via the concentration of Pharmasolve^®.

Figure 2.  Corneal permeability coefficients change of enoxacin via the concentration of Pharmasolve^®.

Figure 3.  Corneal permeability coefficients change of ibuprofen via the concentration of Pharmasolve^®.

Figure 4.  Corneal permeability coefficients change of puerarin via the concentration of Pharmasolve^®.

Table 2.  Effect of Pharmasolve^® on the corneal permeability of test drugs (n = 4).

Drugs	Papp × 10^6 (cm/s)
	0%	2.5%	5%	10%
Ribavirin	5.45 ± 0.87	7.17 ± 0.80	15.21 ± 1.98	22.02 ± 2.78
Puerarin	6.07 ± 0.36	6.70 ± 0.71	8.82 ± 0.83	16.23 ± 1.20
Enoxacin	3.63 ± 0.40	4.87 ± 0.70	12.19 ± 2.55	18.49 ± 3.50
Ibuprofen	29.13 ± 2.33	42.90 ± 4.04	43.99 ± 0.86	46.66 ± 1.84

Data are mean ± SD, n = 4.

Table 3.  Lag-time of drugs in different concentrations of Pharmasolve® (n = 4).

Drugs	Lag-time/(min)
	0%	2.5%	5%	10%
Ribavirin	38.86 ± 8.18	65.56 ± 6.45	39.98 ± 0.48	51.75 ± 1.70
Puerarin	75.51 ± 2.05	69.36 ± 8.83	79.52 ± 2.83	81.33 ± 1.10
Enoxacin	55.97 ± 2.61	84.05 ± 2.39	77.50 ± 2.58	63.52 ± 1.47
Ibuprofen	29.14 ± 10.16	33.45 ± 2.44	44.74 ± 10.37	39.13 ± 6.41

Data are mean ± SD, n = 4.

Table 4.  The corneal hydration of drugs in different concentrations of Pharmasolve^® (n = 4).

Drugs	Corneal hydration %
	0%	2.5%	5%	10%
Ribavirin	77.93 ± 1.74	79.14 ± 2.38	78.26 ± 1.63	78.68 ± 1.94
Puerarin	78.77 ± 2.16	79.93 ± 3.65	77.81 ± 1.20	77.92 ± 0.41
Enoxacin	79.92 ± 1.94	79.53 ± 4.33	76.40 ± 2.22	78.44 ± 1.45
Ibuprofen	81.19 ± 4.80	77.06 ± 1.68	77.03 ± 3.47	77.13 ± 1.46

Data are mean ± SD, n = 4.