Folate receptor mediated intracellular gene delivery using the charge changing solid lipid nanoparticles

Figures & data

Figure 1.  The chemical structure of Chitosan-Foloc Acid conjugation and CHETA.

Table 1.  Zeta potential and size distribution of three different nanoparticles.

	Zeta potential (mV)	Size (nm)	PDI
Folate-chitosan-CHETA-Sln	–40.5 ± 0.8	254.5 ± 5.8	0.253 ± 0.015
Folate-chitosan- Sln	+25.5 ± 0.9	243.8 ± 4.2	0.242 ± 0.012
CHETA-Sln	–50.0 ± 0.6	208.8 ± 6.1	0.192 ± 0.016

Figure 2.  Scanning electron micrograph of Folate-chitosan-CHETA-Sln, scale bar: 1.0mm.

Figure 3.  Zeta potential of folate-chitosan-CHETA−Sln after reaction with different mount of DDT.

Figure 4.  Viability of HEK293 cells determined in the MTT assay under different experimental conditions (15 mg/well): (A) Folate-chitosan-CHETA−Sln; (B) Folate-chitosan-Sln; (C) Cheta−Sln; (D) Lipofectamine^™ 200; (E) Naked DNA. Results are presented as mean ± standard deviation (S.D.) (n = 5).

Figure 5.  The flourescence images of eGFP gene expression in SKOV3 cells (folate receptor over-expressing, R⁺) and A549 cells (folate receptor deficient, R^-) transfected by three different kinds of nanoparticles at the absence of serum. It was observed under the inverse fluorescence microscope (Olympas, japan).

Figure 6.  Transfectivities of Folate-chitosan-CHETA-Sln, Folate-chitosan−Sln, CHETA−Sln and naked DNA in SKOV3 cells (R⁺) and A549 cells (R^-) at the absence of serum. The data represent the mean ± S.D of three wells and was representative of three independent experiments.

Figure 7.  Transfectivities of Folate-chotosan-CHETA−Sln, Folate-chotosan−Sln, CHETA−Sln and naked DNA in SKOV3 cells (R+) at the presence or absence of serum (10%, v/v). The data represent the mean ± SD. of three wells and was representative of three independent experiments.