Research on the comparison of the demethylvancomycin’s diffusion–deposition characteristics in the ocular solid tissues of sustained subtenon drug delivery with subconjunctival injection

Figures & data

Figure 1. A polyvinyl chloride tube was fixed with the suture on the sclera of rabbit (a,b); another end of the tube was connected with an auto-infusion pump to infuse the drug to the subternon (c).

Chart 1. (a) The curve of concentration–time of demethylvancomycin in cornea tissue sequent time points of two groups. (b) The curve of AUC_cum–time of demethylvancomycin in cornea tissues at sequent time points of two groups.

Table 1. The variation of demethylvancomycin concentration in corneal tissue at sequent time points of the two groups (units: μg/g).

	1 h	3 h	6 h	12 h	24 h
Subternon capsular infusion group	51.386 ± 18.653	82.951 ± 7.471	90.442 ± 9.170	63.031 ± 25.149	92.406 ± 21.555	F = 487.775
Subconjunctive injection group	51.778 ± 14.001	0.45 ± 0.26	20.074 ± 4.066	6.116 ± 3.983	6.132 ± 2.473	p < 0.001
	t1 = 0.013	t3 = 18.73	t6 = 15.884	t12 = 5.504	t24 = 9.862
	p = 0.990	p < 0.001	p < 0.001	p = 0.002	p < 0.001

Note: F value was the testing value of one-way analysis of variance to compare the overall difference between the two groups, t values were the testing values of two independent sample t-tests to compare the difference at each sequent time point.

Chart 2. (a) The curve of concentration–time of demethylvancomycin in iris tissues at sequent time points of two groups. (b) The curve of AUC_cum–time of demethylvancomycin in iris tissues at sequent time points of two groups.

Table 2. The variation of demethylvancomycin concentration in iris tissue at sequent time points of the two groups (units: μg/g).

	1 h	3 h	6 h	12 h	24 h
Subternon capsular infusion group	9.759 ± 8.386	27.258 ± 7.153	28.451 ± 10.229	20.046 ± 6.223	16.664 ± 18.078	F = 4.848
Subconjunctive injection group	15.268 ± 4.670	42.271 ± 27.291	9.737 ± 4.238	4.200 ± 2.817	3.333 ± 0.701	p = 0.064
	t1= −1.488	t3= −1.353	t6 = 4.191	t12 = 5.818	t24 = 2.616
	p = 0.171	p = 0.209	p = 0.002	p < 0.001	p = 0.047

Note: F value was the testing value of one-way analysis of variance to compare the overall difference between the two groups, t values were the testing values of two independent sample t-tests to compare the difference at each sequent time point.

Chart 3. (a) The curve of concentration–time of demethylvancomycin in sclera tissues at sequent time points of two groups. (b) The curve of AUC_cum–time of demethylvancomycin in sclera tissues at sequent time points of two groups.

Table 3. The variation of demethylvancomycin concentration in scleral tissue at sequent time points of the two groups (units: μg/g).

	1 h	3 h	6 h	12 h	24 h
Subternon capsular infusion group	30.565 ± 10.070	35.971 ± 10.173	65.457 ± 26.872	90.779 ± 18.942	153.166 ± 51.738	F = 132.748
Subconjunctive injection group	57.423 ± 18.480	32.574 ± 12.037	13.702 ± 4.640	19.798 ± 5.703	15.259 ± 6.386	p < 0.001
	t1= −3.489	t3 = 0.606	t6 = 5.130	t12 = 9.832	t24 = 7.217
	p = 0.006	p = 0.558	p < 0.001	p < 0.001	p < 0.001

Note: F value was the testing value of one-way analysis of variance to compare the overall difference between the two groups, t values were the testing values of two independent sample t-tests to compare the difference at each sequent time point.