Contribution of both olfactory and systemic pathways for brain targeting of nimodipine-loaded lipo-pluronics micelles: in vitro characterization and in vivo biodistribution study after intranasal and intravenous delivery

Figures & data

Figure 1. Variation of the radiochemical Yield of ^99m Tc-NM as a function of NM amount (A), pH (B), Na₂S₂O₄ amount (C), reaction time (D), and temperature (E).

Figure 2. Bar chart showing percentage radioactivity in the heart of mice at different time intervals following administration of radiolabled formulation.

Figure 3. Bar chart showing percentage radioactivity in the brain of mice at different time intervals.

Table 1. The mean pharmacokinetic parameters of NM in blood, and brain of mice after administration of single dose of NM solution and NM-loaded LPM via the IV and IN route.

	IN NM-loaded LPM		IV NM-loaded LPM		IV NM solution
	Blood	Brain	Blood	Brain	Blood	Brain
Cmax (%ID/g)	0.33 ± 0.05	0.51 ± 0.10	24.08 ± 1.73	0.61 ± 0.12	11.30 ± 1.46	0.41 ± 0.09
Tmax (h)	0.58 ± 0.38	0.33 ± 0.14	0.25 ± 0.00	0.33 ± 0.14	0.25 ± 0.00	1.00 ± 0.87
AUC(0–24) (%ID/g h)	2.23 ± 0.20	2.58 ± 0.69	228.91 ± 18.99	5.54 ± 0.23	90.58 ± 8.10	5.60 ± 1.82
t1/2 (h)	11.17 ± 5.07	10.67 ± 5.24	11.68 ± 1.14	14.00 ± 3.86	13.45 ± 2.37	13.40 ± 6.36
MRT (h)	7.62 ± 1.54	7.76 ± 1.57	8.05 ± 0.49	8.49 ± 0.94	8.57 ± 0.08	8.32 ± 0.80

Figure 4. Bar chart showing the brain/blood ratio at different time intervals following administration of radiolabled formulations.