Development of DNA tetrahedron-based drug delivery system

Figures & data

Table 1. Applications and modifications of DNA tetrahedron in the field of drug delivery.

Cargo		Connective approach	Modification	Cell line in vitro	In vivo	Ref.
Small molecules	Doxorubicin	Insetting	L-DNA	SCC7 cells	Y	(Kim et al., 2016)
			Aptamer	MCF-7 cells	N	(Dai et al., 2016)
			Aptamer & Folic acid	HT-29 cells	N	(Sun et al., 2017)
			Tumor-penetrating peptide	U87MG cells	N	(Xia et al., 2016)
			L-DNA	HeLa cells	Y	(Kang et al., 2017)
	Methylene blue	Insetting	/	B16F10 cells SCC7 cells MDA-MB231 cells	Y	(Kim et al., 2016)
	Actinomycin D	Insetting	/	Escherichia coli Staphylococcus aureus	N	(Setyawati et al., 2014)
Nucleic acid	CpG	Pre-Linking	/	RAW264.7 cells	N	(Jiang Li et al., 2011)
	siRNA	Overhang	Folic acid	HeLa cells	Y	(Hyukjin Lee et al., 2012)
	ASOs	Loop	Lipofectamine 2000	Hela cells C2C12 cells MCF7 cells	N	(Keum et al., 2011)
		Insetting	PNA	Escherichia coli	N	(Readman et al., 2017)
	Aptamer	Overhang	/	HeLa cells NIH3T3 cells	N	(Charoenphol & Bermudez, 2014)
		Pre-Linking	L-DNA	HeLa cells NIH-3T3	N	(Kim et al., 2014)
		Overhang	/	MCF-7 cells A549 cells HT-29 cells	N	(Dai et al., 2016)
		Overhang	Folic acid	HT-29 cells	N	(Sun et al., 2017)

Y: yes; N: no.

Figure 1. Modification strategies and intracellular routes of DNA tetrahedron in the process of delivering drugs.

Kim KR, Bang D, Ahn DR. (2016). Nano-formulation of a photosensitizer using a DNA tetrahedron and its potential for in vivo photodynamic therapy. Biomater Sci-UK 4:605–9.

 PubMed Web of Science ®Google Scholar

Dai BD, Hu Y, Duan JH, et al. (2016). Aptamer-guided DNA tetrahedron as a novel targeted drug delivery system for MUC1-expressing breast cancer cells in vitro. Oncotarget 7:38257–69.

 PubMed Web of Science ®Google Scholar

Sun PC, Zhang N, Tang YF, et al. (2017). SL2B aptamer and folic acid dual-targeting DNA nanostructures for synergic biological effect with chemotherapy to combat colorectal cancer. Int J Nanomed 12:2657–72.

 PubMed Web of Science ®Google Scholar

Xia ZW, Wang P, Liu XW, et al. (2016). Tumor-penetrating peptide-modified DNA tetrahedron for targeting drug delivery. Biochemistry 55:1326–31.

 PubMed Web of Science ®Google Scholar

Kang JK, Kim KR, Lee H, et al. (2017). In vitro and in vivo behavior of DNA tetrahedrons as tumor-targeting nanocarriers for doxorubicin delivery. Colloids Surf B Biointerfaces 157:424–31.

 PubMed Web of Science ®Google Scholar

Setyawati MI, Kutty RV, Tay CY, et al. (2014). Novel theranostic DNA nanoscaffolds for the simultaneous detection and killing of Escherichia coli and Staphylococcus aureus. ACS Appl Mater Interfaces 6:21822–31.

 PubMed Web of Science ®Google Scholar

Li J, Pei H, Zhu B, et al. (2011). Self-assembled multivalent DNA nanostructures for noninvasive intracellular delivery of immunostimulatory CpG oligonucleotides. ACS Nano 5:8783–9.

 PubMed Web of Science ®Google Scholar

Lee H, Lytton-Jean AK, Chen Y, et al. (2012). Molecularly self-assembled nucleic acid nanoparticles for targeted in vivo siRNA delivery. Nat Nanotechnol 7:389–93.

 PubMed Web of Science ®Google Scholar

Keum JW, Ahn JH, Bermudez H. (2011). Design, assembly, and activity of antisense DNA nanostructures. Small 7:3529–35.

 PubMed Web of Science ®Google Scholar

Readman JB, Dickson G, Coldham NG. (2017). Tetrahedral DNA nanoparticle vector for intracellular delivery of targeted peptide nucleic acid antisense agents to restore antibiotic sensitivity in cefotaxime-resistant Escherichia coli. Nucleic Acid Ther 27:176–81.

 PubMed Web of Science ®Google Scholar

Charoenphol P, Bermudez H. (2014). Aptamer-targeted DNA nanostructures for therapeutic delivery. Mol Pharmaceut 11:1721–5.

 PubMed Web of Science ®Google Scholar

Kim KR, Lee T, Kim BS, et al. (2014). Utilizing the bioorthogonal base-pairing system of L-DNA to design ideal DNA nanocarriers for enhanced delivery of nucleic acid cargos. Chem Sci 5:1533–7.

 Web of Science ®Google Scholar