Fluconazole-loaded solid lipid nanoparticles topical gel for treatment of pityriasis versicolor: formulation and clinical study

Figures & data

Table 1. Composition of SLNs loaded with Fluconazole and FLZ-SLNs topical gels (% w/w).

	FLZ-SLNs dispersions(% w/w)^a				FLZ-SLNs topical gels (% w/w)^a
Formulation code	Compritol 888 ATO	Precirol ATO5	Poloxamer 407	Cremophor RH40	Carpobol 934	Methyl Paraben	TEA^b	Distilled water Up to	Gel code
SLN 1	8	–	0.5	–	–	–	–	–	–
SLN2	10	–	0.5	–	1	0.1	q.s	100	Gel 1
SLN 3	8	–	–	0.5	–	–	–	–	–
SLN 4	10	–	–	0.5	1	0.1	q.s	100	Gel2
SLN 5	–	8	0.5	–	–	–	–	–	–
SLN 6	–	10	0.5	–	1	0.1	q.s	100	Gel 3
SLN 7	–	8	–	0.5	–	–	–	–	–
SLN 8	–	10	–	0.5	1	0.1	q.s	100	Gel4

^a Fluconazole concentration is 1% w/w.

^b TEA: Triethanolamine.

Table 2. Characterizations of selected FLZ SLNs.

Formulation code	EE%^a	Zeta potential (mv)^a	Polydispersity index^a	Mean Particle Size (nm)^a
SLN1	76.72 ± 1.03	–	–	–
SLN2	82.94 ± 1.24	−21 ± 2.76	0.29 ± 0.64	307 ± 0.32
SLN3	71.27 ± 1.11	–	–	–
SLN4	80.52 ± 1.05	−33.1 ± 1.24	0.255 ± 0.47	500 ± 0.26
SLN5	71.39 ± 1.23	–	–	–
SLN6	79.03 ± 1.11	−22.9 ± 1.66	0.228 ± 0.52	292 ± 0.79
SLN7	55.48 ± 1.21	–	–	–
SLN8	77.04 ± 1.12	−25.7 ± 0.83	0.278 ± 0.79	420 ± 0.53

^a Values are represented as mean ± SD (n = 3).

Figure 1. In vitro release profiles of FLZ from (a) prepared SLNs and (b) relevant SLNs gels.

Figure 2. Mean of healing percentage measured in patients at first, second, third, and fourth week of different treatments application. Capital letters represent the significance of same treatment at different weeks. Small letters represent the significance between different treatments within the same week. Error bars represent standard error (SE).

Figure 3. Photographic pictures before and after treatment with (a) gel 2 and (b) gel 3.

Figure 4. Mycological results representation of gel 2, gel 3, and Candistan^® cream with pairwise comparison at each weak; capital letters represent the significance of same treatment at different weeks and small letters represent the significance between different treatments within the same week.