Adsorption of Lipase on Hollow Fiber Membrane Chips

Figures & data

Figure 1 Microscopic view of a cross section of polysulfone hollow fiber membrane showing the inner thin layers and the spongy outside layer. (From Asahi Medical, Japan)

Figure 2 Changes in the enzyme activity with time for the adsorption of lipase on the polysulfone membrane at 40°C using lipase solution with an initial activity of 333 LU ml⁻¹.

Figure 3 Amount of adsorbed enzyme on the polysulfone membrane at three constant temperatures, 30, 35 and 40°C, using lipase solution of 200 LU ml⁻¹.

Figure 4 Comparison between experimental results and the proposed correlation for the amount of lipase adsorbed, a_ads, versus equilibrium free lipase concentration, a_free. (▪, ♦, and ▴ are experimental results and – is the correlation curve at each temperature.)

Table 1. Langmuir adsorption model parameters

Parameter	Estimated value
α	−3264.7 (LU g^−1)
ε	−3.4 × 10^−3 (K^−1)
Δhads/R	6668.9 (K)
β	4.2 × 10^6 (ml LU^−1)

Figure 5 Effect of adding glutaraldehyde on the amount of lipase adsorbed at initial lipase activity of 500 LU ml⁻¹ and 40°C.